Generation and characterization of the human induced pluripotent stem cell (hiPSC) line NCUFi001-A from a patient carrying KCNQ1 G314S mutation

Generation and characterization of the human induced pluripotent stem cell (hiPSC) line NCUFi001-A from a patient carrying KCNQ1 G314S mutation

In this study we describe the generation and characterization of an human induced pluripotent stem cell (hiPSC) line from a long QT syndrome type 1 (LQT1) patient carrying the KCNQ1 c.940 G > A (p.Gly314Ser) mutation. This patient-specific iPSC line has been obtained by using non-integrational Se...

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Main Authors: Luca Lavra, Fiorenza Magi, Alessandra Ulivieri, Alessandra Morgante, Marianna Paulis, Luca Sala, Matteo Pedrazzini, Patrizio Polisca, Marcella Rocchetti, Leonardo Calò, Salvatore Sciacchitano, Leila B. Salehi
Format: Article
Language:English
Published: Elsevier 2021-07-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506121002646
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spelling doaj-3dee8cd02ef6407b96a8a6deaf2c10672021-07-09T04:42:39ZengElsevierStem Cell Research1873-50612021-07-0154102418Generation and characterization of the human induced pluripotent stem cell (hiPSC) line NCUFi001-A from a patient carrying KCNQ1 G314S mutationLuca Lavra0Fiorenza Magi1Alessandra Ulivieri2Alessandra Morgante3Marianna Paulis4Luca Sala5Matteo Pedrazzini6Patrizio Polisca7Marcella Rocchetti8Leonardo Calò9Salvatore Sciacchitano10Leila B. Salehi11Laboratory of Biomedical Research, Niccolò Cusano University Foundation, Via Don Carlo Gnocchi 3, 00166 Rome, Italy; Corresponding author.Laboratory of Biomedical Research, Niccolò Cusano University Foundation, Via Don Carlo Gnocchi 3, 00166 Rome, ItalyLaboratory of Biomedical Research, Niccolò Cusano University Foundation, Via Don Carlo Gnocchi 3, 00166 Rome, ItalyLaboratory of Biomedical Research, Niccolò Cusano University Foundation, Via Don Carlo Gnocchi 3, 00166 Rome, ItalyHumanitas Clinical and Research Center-IRCCS, Rozzano, 20089 Milan, Italy; Institute of Genetic and Biomedical Research (IRGB)-UOS of Milan, National Research Council (CNR), 20138 Milan, ItalyCenter for Cardiac Arrhythmias of Genetic Origin and Laboratory of Cardiovascular Genetics, Istituto Auxologico Italiano IRCCS, Via Zucchi 18, Cusano Milanino, MI, ItalyCenter for Cardiac Arrhythmias of Genetic Origin and Laboratory of Cardiovascular Genetics, Istituto Auxologico Italiano IRCCS, Via Zucchi 18, Cusano Milanino, MI, ItalyLaboratory of Biomedical Research, Niccolò Cusano University Foundation, Via Don Carlo Gnocchi 3, 00166 Rome, Italy; Department of Biomedicine and Prevention, University of Rome Tor Vergata, Viale Montpellier 1, 00133 Rome, ItalyDepartment of Biotechnology and Biosciences, Università degli Studi di Milano-Bicocca, Piazza della Scienza 2, 20126 Milan, ItalyDivision of Cardiology, Policlinico Casilino, Via Casilina 1049, 00169 Rome, ItalyLaboratory of Biomedical Research, Niccolò Cusano University Foundation, Via Don Carlo Gnocchi 3, 00166 Rome, Italy; Department of Clinical and Molecular Medicine, Sapienza University, Via di Grottarossa 1035/1039, 00189 Rome, ItalyLaboratory of Biomedical Research, Niccolò Cusano University Foundation, Via Don Carlo Gnocchi 3, 00166 Rome, Italy; Medical Genetics Unit, Tor Vergata University Hospital, PTV, Viale Montpellier 1, 00133 Rome, ItalyIn this study we describe the generation and characterization of an human induced pluripotent stem cell (hiPSC) line from a long QT syndrome type 1 (LQT1) patient carrying the KCNQ1 c.940 G > A (p.Gly314Ser) mutation. This patient-specific iPSC line has been obtained by using non-integrational Sendai reprogramming method, expresses pluripotency markers and has the capacity to differentiate into the three germ layers and into spontaneously beating cardiomyocytes (iPSC-CMs).http://www.sciencedirect.com/science/article/pii/S1873506121002646
collection DOAJ
language English
format Article
sources DOAJ
author Luca Lavra
Fiorenza Magi
Alessandra Ulivieri
Alessandra Morgante
Marianna Paulis
Luca Sala
Matteo Pedrazzini
Patrizio Polisca
Marcella Rocchetti
Leonardo Calò
Salvatore Sciacchitano
Leila B. Salehi
spellingShingle Luca Lavra
Fiorenza Magi
Alessandra Ulivieri
Alessandra Morgante
Marianna Paulis
Luca Sala
Matteo Pedrazzini
Patrizio Polisca
Marcella Rocchetti
Leonardo Calò
Salvatore Sciacchitano
Leila B. Salehi
Generation and characterization of the human induced pluripotent stem cell (hiPSC) line NCUFi001-A from a patient carrying KCNQ1 G314S mutation
Stem Cell Research
author_facet Luca Lavra
Fiorenza Magi
Alessandra Ulivieri
Alessandra Morgante
Marianna Paulis
Luca Sala
Matteo Pedrazzini
Patrizio Polisca
Marcella Rocchetti
Leonardo Calò
Salvatore Sciacchitano
Leila B. Salehi
author_sort Luca Lavra
title Generation and characterization of the human induced pluripotent stem cell (hiPSC) line NCUFi001-A from a patient carrying KCNQ1 G314S mutation
title_short Generation and characterization of the human induced pluripotent stem cell (hiPSC) line NCUFi001-A from a patient carrying KCNQ1 G314S mutation
title_full Generation and characterization of the human induced pluripotent stem cell (hiPSC) line NCUFi001-A from a patient carrying KCNQ1 G314S mutation
title_fullStr Generation and characterization of the human induced pluripotent stem cell (hiPSC) line NCUFi001-A from a patient carrying KCNQ1 G314S mutation
title_full_unstemmed Generation and characterization of the human induced pluripotent stem cell (hiPSC) line NCUFi001-A from a patient carrying KCNQ1 G314S mutation
title_sort generation and characterization of the human induced pluripotent stem cell (hipsc) line ncufi001-a from a patient carrying kcnq1 g314s mutation
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2021-07-01
description In this study we describe the generation and characterization of an human induced pluripotent stem cell (hiPSC) line from a long QT syndrome type 1 (LQT1) patient carrying the KCNQ1 c.940 G > A (p.Gly314Ser) mutation. This patient-specific iPSC line has been obtained by using non-integrational Sendai reprogramming method, expresses pluripotency markers and has the capacity to differentiate into the three germ layers and into spontaneously beating cardiomyocytes (iPSC-CMs).
url http://www.sciencedirect.com/science/article/pii/S1873506121002646
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