Controlling Ratios of Plasmid-Based Double Cut Donor and CRISPR/Cas9 Components to Enhance Targeted Integration of Transgenes in Chinese Hamster Ovary Cells

Controlling Ratios of Plasmid-Based Double Cut Donor and CRISPR/Cas9 Components to Enhance Targeted Integration of Transgenes in Chinese Hamster Ovary Cells

Chinese hamster ovary (CHO) cells are the most valuable expression host for the commercial production of biotherapeutics. Recent trends in recombinant CHO cell-line development have focused on the site-specific integration of transgenes encoding recombinant proteins over random integration. However,...

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Bibliographic Details
Main Authors: Sung Wook Shin, Dongwoo Kim, Jae Seong Lee
Format: Article
Language:English
Published: MDPI AG 2021-02-01
Series:International Journal of Molecular Sciences
Subjects:
Chinese hamster ovary (CHO)
CRISPR/Cas9
Double cut donor
Homology-directed repair
Multiple knock-in
Targeted integration
Online Access:https://www.mdpi.com/1422-0067/22/5/2407

Internet

https://www.mdpi.com/1422-0067/22/5/2407

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