Gene transfer to primary corneal epithelial cells with an integrating lentiviral vector

Gene transfer to primary corneal epithelial cells with an integrating lentiviral vector

PURPOSE: To evaluate the transfer of heterologous genes carrying a Green Fluorescent Protein (GFP) reporter cassette to primary corneal epithelial cells ex vivo. METHODS: Freshly enucleated rabbit corneoscleral tissue was used to obtain corneal epithelial cell suspension via enzymatic digestion. Cel...

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Bibliographic Details
Main Authors: Lauro Augusto de Oliveira, Charles Kim, Luciene Barbosa de Sousa, Ivan R. Schwab, Mark I. Rosenblatt
Format: Article
Language:English
Published: Conselho Brasileiro de Oftalmologia 2010-10-01
Series:Arquivos Brasileiros de Oftalmologia
Subjects:
Terapia de genes
Doenças da córnea
Lentivírus
Trangenes
Células tronco
Epitélio anterior
Expressão gênica
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492010000500012&lng=en&tlng=en

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