DETERMINATION OF T-2 TOXIN BY THE IMMUNOENZYME AND IMMUNOSENSOR METHODS

• Main Authors: О. S. Gojster, W. Е. Kriwenchuk, G. O. Chmelnitsky, О. Н. Minchenko
• Format: Article
• Language: English
• Published: National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. 2013-10-01
• Series: Biotechnologia Acta
• Subjects: T-2 toxin; conjugates; antibody; immune enzymatic analysis; immune biosensor
• Online Access: http://biotechnology.kiev.ua/images/storage/no5_2013/goister_5_2013.pdf

The aim of this work was the development of T-2 toxin immune-detection method using biosensor approaches. For this aim the conjugates of T-2 toxin with proteins were obtained and used for antibody preparation. The specific properties of obtained antibodies were studied by immunochemical methods. It was shown that carrier enzyme plays important role in realization of immunogenic properties of T-2 hapten. The detection time of IgG fraction as well as antiserum binding activity by immunoenzyme and immunosensor analyses is 10–15 minutes. Two variants of T-2 toxin determination in competitive mode of immunochemical analysis were suggested, i. e. ELISA and immunosensor method (based on surface plasmon resonance phenomenon) with detection from 10 ng/ml and 1 ng/ml, correspondingly. These methods can be applied for detection of T-2 toxin in the food and fodder in concentration from 1 up to 500 ng/ml.