Isolation of Human CD138+ Microparticles from the Plasma of Patients with Multiple Myeloma

The confinement of multiple myeloma (MM) to the bone marrow microenvironment requires an invasive bone marrow biopsy to monitor the malignant compartment. The existing clinical tools used to determine treatment response and tumor relapse are limited in sensitivity mainly because they indirectly meas...

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Main Authors: Sabna Rajeev Krishnan, Frederick Luk, Ross D Brown, Hayley Suen, Yiulam Kwan, Mary Bebawy
Format: Article
Language:English
Published: Elsevier 2016-01-01
Series:Neoplasia: An International Journal for Oncology Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1476558615001566
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spelling doaj-3d61214c6dde4504b2b92224cbc437d52020-11-25T00:44:08ZengElsevierNeoplasia: An International Journal for Oncology Research1476-55861522-80022016-01-01181253210.1016/j.neo.2015.11.011Isolation of Human CD138+ Microparticles from the Plasma of Patients with Multiple MyelomaSabna Rajeev Krishnan0Frederick Luk1Ross D Brown2Hayley Suen3Yiulam Kwan4Mary Bebawy5Graduate School of Health, Discipline of Pharmacy, University of Technology Sydney, NSW 2007, AustraliaGraduate School of Health, Discipline of Pharmacy, University of Technology Sydney, NSW 2007, AustraliaInstitute of Haematology, Royal Prince Alfred Hospital, Camperdown, NSW 2050, AustraliaInstitute of Haematology, Royal Prince Alfred Hospital, Camperdown, NSW 2050, AustraliaDepartment of Haematology, Concord Repatriation General Hospital, Concord, NSW 2139, AustraliaGraduate School of Health, Discipline of Pharmacy, University of Technology Sydney, NSW 2007, AustraliaThe confinement of multiple myeloma (MM) to the bone marrow microenvironment requires an invasive bone marrow biopsy to monitor the malignant compartment. The existing clinical tools used to determine treatment response and tumor relapse are limited in sensitivity mainly because they indirectly measure tumor burden inside the bone marrow and fail to capture the patchy, multisite tumor infiltrates associated with MM. Microparticles (MPs) are 0.1- to 1.0-μm membrane vesicles, which contain the cellular content of their originating cell. MPs are functional mediators and convey prothrombotic, promalignant, proresistance, and proinflammatory messages, establishing intercellular cross talk and bypassing the need for direct cell-cell contact in many pathologies. In this study, we analyzed plasma cell–derived MPs (CD138+) from deidentified MM patients (n = 64) and normal subjects (n = 18) using flow cytometry. The morphology and size of the MPs were further analyzed using scanning electron microscopy. Our study shows the proof of a systemic signature of MPs in MM patients. We observed that the levels of MPs were significantly elevated in MM corresponding to the tumor burden. We provide the first evidence for the presence of MPs in the peripheral blood of MM patients with potential applications in personalized MM clinical monitoring.http://www.sciencedirect.com/science/article/pii/S1476558615001566
collection DOAJ
language English
format Article
sources DOAJ
author Sabna Rajeev Krishnan
Frederick Luk
Ross D Brown
Hayley Suen
Yiulam Kwan
Mary Bebawy
spellingShingle Sabna Rajeev Krishnan
Frederick Luk
Ross D Brown
Hayley Suen
Yiulam Kwan
Mary Bebawy
Isolation of Human CD138+ Microparticles from the Plasma of Patients with Multiple Myeloma
Neoplasia: An International Journal for Oncology Research
author_facet Sabna Rajeev Krishnan
Frederick Luk
Ross D Brown
Hayley Suen
Yiulam Kwan
Mary Bebawy
author_sort Sabna Rajeev Krishnan
title Isolation of Human CD138+ Microparticles from the Plasma of Patients with Multiple Myeloma
title_short Isolation of Human CD138+ Microparticles from the Plasma of Patients with Multiple Myeloma
title_full Isolation of Human CD138+ Microparticles from the Plasma of Patients with Multiple Myeloma
title_fullStr Isolation of Human CD138+ Microparticles from the Plasma of Patients with Multiple Myeloma
title_full_unstemmed Isolation of Human CD138+ Microparticles from the Plasma of Patients with Multiple Myeloma
title_sort isolation of human cd138+ microparticles from the plasma of patients with multiple myeloma
publisher Elsevier
series Neoplasia: An International Journal for Oncology Research
issn 1476-5586
1522-8002
publishDate 2016-01-01
description The confinement of multiple myeloma (MM) to the bone marrow microenvironment requires an invasive bone marrow biopsy to monitor the malignant compartment. The existing clinical tools used to determine treatment response and tumor relapse are limited in sensitivity mainly because they indirectly measure tumor burden inside the bone marrow and fail to capture the patchy, multisite tumor infiltrates associated with MM. Microparticles (MPs) are 0.1- to 1.0-μm membrane vesicles, which contain the cellular content of their originating cell. MPs are functional mediators and convey prothrombotic, promalignant, proresistance, and proinflammatory messages, establishing intercellular cross talk and bypassing the need for direct cell-cell contact in many pathologies. In this study, we analyzed plasma cell–derived MPs (CD138+) from deidentified MM patients (n = 64) and normal subjects (n = 18) using flow cytometry. The morphology and size of the MPs were further analyzed using scanning electron microscopy. Our study shows the proof of a systemic signature of MPs in MM patients. We observed that the levels of MPs were significantly elevated in MM corresponding to the tumor burden. We provide the first evidence for the presence of MPs in the peripheral blood of MM patients with potential applications in personalized MM clinical monitoring.
url http://www.sciencedirect.com/science/article/pii/S1476558615001566
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