Study of enzymatic properties of Chitsan-immobilized Alcohol Dehydrogenase

• Main Authors: LI Xue, ZHANG Caiyan, TONG Kaichen, MA Zhongliang
• Format: Article
• Language: English
• Published: Academic Journals Center of Shanghai Normal University 2016-08-01
• Series: Journal of Shanghai Normal University (Natural Sciences)
• Subjects: chitosan; alcohol dehydrogenases; immobilized enzyme
• Online Access: http://qktg.shnu.edu.cn/zrb/shsfqkszrb/ch/reader/create_pdf.aspx?file_no=201604015&flag=1&year_id=2016&quarter_id=4

Taking free enzyme as control,we investigated the enzymatic properties of immobilized alcohol dehydrogenase using chitosan as carrier and glutaraldehyde as crosslinking agent.The results showed that the resistance of immobilized alcohol dehydrogenase to temperature,alkaline environment and Cu²⁺ was significantly improved when the chitosan was crosslinked with 0.6% glutaraldehyde for 6 hours,and the operation stability was also obviously improved.