Differentially expressed genes in embryonic cardiac tissues of mice lacking <it>Folr1 </it>gene activity

• Main Authors: Schwartz Robert J, Wang Deli, Bozinov Daniel, Wlodarczyk Bogdan J, Cabrera Robert M, Zhu Huiping, Finnell Richard H
• Format: Article
• Language: English
• Published: BMC 2007-11-01
• Series: BMC Developmental Biology
• Online Access: http://www.biomedcentral.com/1471-213X/7/128

<p>Abstract</p> <p>Background</p> <p>Heart anomalies are the most frequently observed among all human congenital defects. As with the situation for neural tube defects (NTDs), it has been demonstrated that women who use multivitamins containing folic acid peri-conceptionally have a reduced risk for delivering offspring with conotruncal heart defects <abbrgrp><abbr bid="B1">1</abbr><abbr bid="B2">2</abbr><abbr bid="B3">3</abbr></abbrgrp>. Cellular folate transport is mediated by a receptor or binding protein and by an anionic transporter protein system. Defective function of the <it>Folr1 </it>(also known as <it>Folbp1</it>; homologue of human <it>FRα</it>) gene in mice results in inadequate transport, accumulation, or metabolism of folate during cardiovascular morphogenesis.</p> <p>Results</p> <p>We have observed cardiovascular abnormalities including outflow tract and aortic arch arterial defects in genetically compromised <it>Folr1 </it>knockout mice. In order to investigate the molecular mechanisms underlying the failure to complete development of outflow tract and aortic arch arteries in the <it>Folr1 </it>knockout mouse model, we examined tissue-specific gene expression difference between <it>Folr1 </it>nullizygous embryos and morphologically normal heterozygous embryos during early cardiac development (14-somite stage), heart tube looping (28-somite stage), and outflow track septation (38-somite stage). Microarray analysis was performed as a primary screening, followed by investigation using quantitative real-time PCR assays. Gene ontology analysis highlighted the following ontology groups: cell migration, cell motility and localization of cells, structural constituent of cytoskeleton, cell-cell adhesion, oxidoreductase, protein folding and mRNA processing. This study provided preliminary data and suggested potential candidate genes for further description and investigation.</p> <p>Conclusion</p> <p>The results suggested that <it>Folr1 </it>gene ablation and abnormal folate homeostasis altered gene expression in developing heart and conotruncal tissues. These changes affected normal cytoskeleton structures, cell migration and motility as well as cellular redox status, which may contribute to cardiovascular abnormalities in mouse embryos lacking <it>Folr1 </it>gene activity.</p>