A novel quantitative fluorescent reporter assay for RAG targets and RAG activity

A novel quantitative fluorescent reporter assay for RAG targets and RAG activity

Recombination-Activating Genes (RAG) 1 and 2 form the site specific recombinase that mediates V(D)J recombination, a process of DNA editing required for lymphocyte development and responsible for their diverse repertoire of antigen receptors. Mistargeted RAG activity associates with genome alteratio...

Full description

Bibliographic Details
Main Authors:	Ines eTrancoso, Marie eBonnet, Rui eGardner, Jorge eCarneiro, Vasco M Barreto, Jocelyne eDemengeot, Leonor M Sarmento
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2013-05-01
Series:	Frontiers in Immunology
Subjects:	Green Fluorescent Proteins Humans Leukemia Lymphocytes Plasmids Recombination, Genetic
Online Access:	http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00110/full

Similar Items

Genome-Wide Analysis Identifies Rag1 and Rag2 as Novel Notch1 Transcriptional Targets in Thymocytes
by: Yang Dong, et al.
Published: (2021-07-01)

PRDM14 promotes RAG-dependent Notch1 driver mutations in mouse T-ALL
by: Brandi L. Carofino, et al.
Published: (2016-05-01)

Sequence of the RAG1 and RAG2 Intergenic Region in Zebrafish (Danio rerio)
by: Bertrand III, F. E., et al.
Published: (2015)

Mechanism of RAG Regulation During Its Physiological and Pathological Functions in Lymphoid Cells
by: Kumari, Rupa
Published: (2018)

Sequence of the RAG1 and RAG2 Intergenic Region inZebrafish (Danio rerio)
by: F. E. Bertrand III, et al.
Published: (1998-01-01)

Adapting to a changing world: <it>RAG </it>genomics and evolution
by: de Camargo Maristela, et al.
Published: (2005-06-01)

Bill costley's rag(a)s Bill costley's rag(a)s
by: Rita Balzar
Published: (2008-04-01)

Studies of the structure and function of the RAG-1 and RAG-2 genes
by: Silver, Daniel P. (Daniel Peter)
Published: (2005)

Identification of RAG-like transposons in protostomes suggests their ancient bilaterian origin
by: Eliza C. Martin, et al.
Published: (2020-05-01)

RagA, but Not RagB, Is Essential for Embryonic Development and Adult Mice
by: Kirak, Oktay, et al.
Published: (2017)

A new take on V(D)J recombination: transcription driven nuclear and chromatin reorganization in RAG–mediated cleavage.
by: Julie eChaumeil, et al.
Published: (2013-12-01)

Rag and Bone: Poems
by: Nuernberger, Kathryn L.
Published: (2011)

What makes a RAG regeneration associated?
by: Thong C Ma, et al.
Published: (2015-08-01)

Modeling of the RAG Reaction Mechanism
by: Amjad Askary, et al.
Published: (2014-04-01)

What's ragged should be left ragged : a Wittgensteinian investigation into the 'messiness' of religious beliefs and utterances
by: Citron, Gabriel
Published: (2012)

Sequence Analysis of the Mouse RAG Locus lntergenic Region
by: F. E. Bertrand III, et al.
Published: (1998-01-01)

RAG2 and XLF/Cernunnos interplay reveals a novel role for the RAG complex in DNA repair
by: Chloé Lescale, et al.
Published: (2016-02-01)

Molecular Diagnosis of Myoclonus Epilepsy Associated with Ragged-Red Fibers Syndrome in the Absence of Ragged Red Fibers
by: Sun Yeong Park, et al.
Published: (2017-09-01)

Rag defects and thymic stroma: lessons from animal models
by: Veronica eMarrella, et al.
Published: (2014-06-01)

Mechanism Of RAG Action As A Structure-Specific Nuclease : Implications In Genomic Instability In Lymphoid Cells
by: Naik, Abani Kanta
Published: (2013)

Construction of <i>Yersinia pestis</i> Strain Producing Fluorescent Protein GFP and Prospects of Its Usage
by: L. M. Kukleva, et al.
Published: (2020-01-01)

RAG: An update to the RNA-As-Graphs resource
by: Elmetwaly Shereef, et al.
Published: (2011-05-01)

RAG: RNA-As-Graphs web resource
by: Laserson Uri, et al.
Published: (2004-07-01)

Menkes Disease with ‘Ragged Red’ Fibers
by: J Gordon Millichap
Published: (1989-01-01)

The Architecture of the Rag GTPase Signaling Network
by: Raffaele Nicastro, et al.
Published: (2017-06-01)

Polymorphism in the rag locus of porphyromonas gingivalis
by: Fawell, Stuart Colin
Published: (2004)

Functional regulation of an ancestral RAG transposon ProtoRAG by a trans-acting factor YY1 in lancelet
by: Song Liu, et al.
Published: (2020-09-01)

A “Real Time” PCR Assay to Detect Transplanted Human Liver Cells in RAG-1-/- Mice
by: Ann W. Funkhouser M.D., et al.
Published: (2001-01-01)

Corrigendum: Rag defects and thymic stroma: lessons from animal models
by: Anna eVilla
Published: (2014-08-01)

iRAGu: A Novel Inducible and Reversible Mouse Model for Ubiquitous Recombinase Activity
by: Marie Bonnet, et al.
Published: (2017-11-01)

A Stylistic Survey and Performance Guide of Three Popular Rags and Three Rags, Composed after 2008 by William Bolcom
Published: (2020)

Rag1 and Rag2 Gene Expressions Identify Lymphopoietic Tissues in Larvae of Rice-Field Eel (Monopterus albus)
by: Fan, Y., et al.
Published: (2022)

Structural Analysis of the TOL pDK1 xylGFJQK Region and Partial Characterization of the xylF and xylG Gene Products
by: Poulter, Melinda D.
Published: (1999)

Characterization of Genetic Structures of the QepA3 Gene in Clinical Isolates of Enterobacteriaceae
by: Dongguo eWang, et al.
Published: (2015-10-01)

Molecular Insights into Lymphoid Malignancy : Role of Transcription Factor BCL11B in T-cell Leukemia Genesis and Biochemical Characterization of DNA Binding Domain of RAG1
by: Deepthi, R
Published: (2018)

Impaired sense of smell and altered olfactory system in RAG-1-/- immunodeficient mice
by: Lorenza eRattazzi, et al.
Published: (2015-09-01)

Spectral sensitivity of the discoloration of Historical rag paper
by: Yun Liu, et al.
Published: (2021-12-01)

Characterization of <it>rag</it>1 mutant zebrafish leukocytes
by: Hanson Larry, et al.
Published: (2009-02-01)

An improved process for the manufacture of rag half stock
by: Kukolich, Stephen Irvin
Published: (2005)

A Fragile Dignity Despite Their Rags and Tatters
by: Byron Breedlove
Published: (2014-10-01)