Single-cell characterization of in vitro migration and interaction dynamics of T cells expanded with IL-2 and IL-7

T cells are pivotal in the immune defense against cancers and infectious agents. To mount an effector response against cancer cells, T cells need to migrate to the cancer-site, engage in contacts with cancer cells and perform their effector functions. Adoptive T cell therapy is an effective strategy...

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Main Authors: Johanna Maria Tauriainen, Karin eGustafsson, Mårten eGöthlin, Jens eGertow, Marcus eBuggert, Thomas Wilhelm Frisk, Annika C Karlsson, Michael eUhlin, Björn eÖnfelt
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-04-01
Series:Frontiers in Immunology
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00196/full
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spelling doaj-3d36296ff0d94355830dd8c1f40324d72020-11-25T01:58:24ZengFrontiers Media S.A.Frontiers in Immunology1664-32242015-04-01610.3389/fimmu.2015.00196121974Single-cell characterization of in vitro migration and interaction dynamics of T cells expanded with IL-2 and IL-7Johanna Maria Tauriainen0Karin eGustafsson1Mårten eGöthlin2Jens eGertow3Marcus eBuggert4Thomas Wilhelm Frisk5Annika C Karlsson6Michael eUhlin7Björn eÖnfelt8Björn eÖnfelt9Karolinska InstitutetKTH-Royal Institute of TechnologyKarolinska InstitutetKarolinska University HospitalKarolinska InstitutetKTH-Royal Institute of TechnologyKarolinska InstitutetKarolinska University HospitalKarolinska InstitutetKTH-Royal Institute of TechnologyT cells are pivotal in the immune defense against cancers and infectious agents. To mount an effector response against cancer cells, T cells need to migrate to the cancer-site, engage in contacts with cancer cells and perform their effector functions. Adoptive T cell therapy is an effective strategy as treatment of complications such as relapse or opportunistic infections after hematopoietic stem cell transplantations. This requires a sufficient amount of cells that are able to expand and respond to tumor or viral antigens. The cytokines interleukin (IL)-2 and IL-7 drive T cell differentiation, proliferation and survival and are commonly used to expand T cells ex vivo. Here, we have used microchip-based live-cell imaging to follow the migration of individual T cells, their interactions with allogeneic monocytes, cell division and apoptosis for extended periods of time; something that cannot be achieved by commonly used methods. Our data indicate that cells grown in IL-7 + IL-2 had similar migration and contact dynamics as cells grown in IL-2 alone. However, the addition of IL-7 decreased cell death creating a more viable cell population, which should be beneficial when preparing cells for immunotherapy.http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00196/fullMicroscopy, FluorescenceSingle-Cell AnalysisT cellIL-2IL-7live-cell imaging
collection DOAJ
language English
format Article
sources DOAJ
author Johanna Maria Tauriainen
Karin eGustafsson
Mårten eGöthlin
Jens eGertow
Marcus eBuggert
Thomas Wilhelm Frisk
Annika C Karlsson
Michael eUhlin
Björn eÖnfelt
Björn eÖnfelt
spellingShingle Johanna Maria Tauriainen
Karin eGustafsson
Mårten eGöthlin
Jens eGertow
Marcus eBuggert
Thomas Wilhelm Frisk
Annika C Karlsson
Michael eUhlin
Björn eÖnfelt
Björn eÖnfelt
Single-cell characterization of in vitro migration and interaction dynamics of T cells expanded with IL-2 and IL-7
Frontiers in Immunology
Microscopy, Fluorescence
Single-Cell Analysis
T cell
IL-2
IL-7
live-cell imaging
author_facet Johanna Maria Tauriainen
Karin eGustafsson
Mårten eGöthlin
Jens eGertow
Marcus eBuggert
Thomas Wilhelm Frisk
Annika C Karlsson
Michael eUhlin
Björn eÖnfelt
Björn eÖnfelt
author_sort Johanna Maria Tauriainen
title Single-cell characterization of in vitro migration and interaction dynamics of T cells expanded with IL-2 and IL-7
title_short Single-cell characterization of in vitro migration and interaction dynamics of T cells expanded with IL-2 and IL-7
title_full Single-cell characterization of in vitro migration and interaction dynamics of T cells expanded with IL-2 and IL-7
title_fullStr Single-cell characterization of in vitro migration and interaction dynamics of T cells expanded with IL-2 and IL-7
title_full_unstemmed Single-cell characterization of in vitro migration and interaction dynamics of T cells expanded with IL-2 and IL-7
title_sort single-cell characterization of in vitro migration and interaction dynamics of t cells expanded with il-2 and il-7
publisher Frontiers Media S.A.
series Frontiers in Immunology
issn 1664-3224
publishDate 2015-04-01
description T cells are pivotal in the immune defense against cancers and infectious agents. To mount an effector response against cancer cells, T cells need to migrate to the cancer-site, engage in contacts with cancer cells and perform their effector functions. Adoptive T cell therapy is an effective strategy as treatment of complications such as relapse or opportunistic infections after hematopoietic stem cell transplantations. This requires a sufficient amount of cells that are able to expand and respond to tumor or viral antigens. The cytokines interleukin (IL)-2 and IL-7 drive T cell differentiation, proliferation and survival and are commonly used to expand T cells ex vivo. Here, we have used microchip-based live-cell imaging to follow the migration of individual T cells, their interactions with allogeneic monocytes, cell division and apoptosis for extended periods of time; something that cannot be achieved by commonly used methods. Our data indicate that cells grown in IL-7 + IL-2 had similar migration and contact dynamics as cells grown in IL-2 alone. However, the addition of IL-7 decreased cell death creating a more viable cell population, which should be beneficial when preparing cells for immunotherapy.
topic Microscopy, Fluorescence
Single-Cell Analysis
T cell
IL-2
IL-7
live-cell imaging
url http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00196/full
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