Single-cell characterization of in vitro migration and interaction dynamics of T cells expanded with IL-2 and IL-7
T cells are pivotal in the immune defense against cancers and infectious agents. To mount an effector response against cancer cells, T cells need to migrate to the cancer-site, engage in contacts with cancer cells and perform their effector functions. Adoptive T cell therapy is an effective strategy...
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doaj-3d36296ff0d94355830dd8c1f40324d72020-11-25T01:58:24ZengFrontiers Media S.A.Frontiers in Immunology1664-32242015-04-01610.3389/fimmu.2015.00196121974Single-cell characterization of in vitro migration and interaction dynamics of T cells expanded with IL-2 and IL-7Johanna Maria Tauriainen0Karin eGustafsson1Mårten eGöthlin2Jens eGertow3Marcus eBuggert4Thomas Wilhelm Frisk5Annika C Karlsson6Michael eUhlin7Björn eÖnfelt8Björn eÖnfelt9Karolinska InstitutetKTH-Royal Institute of TechnologyKarolinska InstitutetKarolinska University HospitalKarolinska InstitutetKTH-Royal Institute of TechnologyKarolinska InstitutetKarolinska University HospitalKarolinska InstitutetKTH-Royal Institute of TechnologyT cells are pivotal in the immune defense against cancers and infectious agents. To mount an effector response against cancer cells, T cells need to migrate to the cancer-site, engage in contacts with cancer cells and perform their effector functions. Adoptive T cell therapy is an effective strategy as treatment of complications such as relapse or opportunistic infections after hematopoietic stem cell transplantations. This requires a sufficient amount of cells that are able to expand and respond to tumor or viral antigens. The cytokines interleukin (IL)-2 and IL-7 drive T cell differentiation, proliferation and survival and are commonly used to expand T cells ex vivo. Here, we have used microchip-based live-cell imaging to follow the migration of individual T cells, their interactions with allogeneic monocytes, cell division and apoptosis for extended periods of time; something that cannot be achieved by commonly used methods. Our data indicate that cells grown in IL-7 + IL-2 had similar migration and contact dynamics as cells grown in IL-2 alone. However, the addition of IL-7 decreased cell death creating a more viable cell population, which should be beneficial when preparing cells for immunotherapy.http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00196/fullMicroscopy, FluorescenceSingle-Cell AnalysisT cellIL-2IL-7live-cell imaging |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Johanna Maria Tauriainen Karin eGustafsson Mårten eGöthlin Jens eGertow Marcus eBuggert Thomas Wilhelm Frisk Annika C Karlsson Michael eUhlin Björn eÖnfelt Björn eÖnfelt |
spellingShingle |
Johanna Maria Tauriainen Karin eGustafsson Mårten eGöthlin Jens eGertow Marcus eBuggert Thomas Wilhelm Frisk Annika C Karlsson Michael eUhlin Björn eÖnfelt Björn eÖnfelt Single-cell characterization of in vitro migration and interaction dynamics of T cells expanded with IL-2 and IL-7 Frontiers in Immunology Microscopy, Fluorescence Single-Cell Analysis T cell IL-2 IL-7 live-cell imaging |
author_facet |
Johanna Maria Tauriainen Karin eGustafsson Mårten eGöthlin Jens eGertow Marcus eBuggert Thomas Wilhelm Frisk Annika C Karlsson Michael eUhlin Björn eÖnfelt Björn eÖnfelt |
author_sort |
Johanna Maria Tauriainen |
title |
Single-cell characterization of in vitro migration and interaction dynamics of T cells expanded with IL-2 and IL-7 |
title_short |
Single-cell characterization of in vitro migration and interaction dynamics of T cells expanded with IL-2 and IL-7 |
title_full |
Single-cell characterization of in vitro migration and interaction dynamics of T cells expanded with IL-2 and IL-7 |
title_fullStr |
Single-cell characterization of in vitro migration and interaction dynamics of T cells expanded with IL-2 and IL-7 |
title_full_unstemmed |
Single-cell characterization of in vitro migration and interaction dynamics of T cells expanded with IL-2 and IL-7 |
title_sort |
single-cell characterization of in vitro migration and interaction dynamics of t cells expanded with il-2 and il-7 |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2015-04-01 |
description |
T cells are pivotal in the immune defense against cancers and infectious agents. To mount an effector response against cancer cells, T cells need to migrate to the cancer-site, engage in contacts with cancer cells and perform their effector functions. Adoptive T cell therapy is an effective strategy as treatment of complications such as relapse or opportunistic infections after hematopoietic stem cell transplantations. This requires a sufficient amount of cells that are able to expand and respond to tumor or viral antigens. The cytokines interleukin (IL)-2 and IL-7 drive T cell differentiation, proliferation and survival and are commonly used to expand T cells ex vivo. Here, we have used microchip-based live-cell imaging to follow the migration of individual T cells, their interactions with allogeneic monocytes, cell division and apoptosis for extended periods of time; something that cannot be achieved by commonly used methods. Our data indicate that cells grown in IL-7 + IL-2 had similar migration and contact dynamics as cells grown in IL-2 alone. However, the addition of IL-7 decreased cell death creating a more viable cell population, which should be beneficial when preparing cells for immunotherapy. |
topic |
Microscopy, Fluorescence Single-Cell Analysis T cell IL-2 IL-7 live-cell imaging |
url |
http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00196/full |
work_keys_str_mv |
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