Pressure-driven release of viral genome into a host nucleus is a mechanism leading to herpes infection
Many viruses previously have been shown to have pressurized genomes inside their viral protein shell, termed the capsid. This pressure results from the tight confinement of negatively charged viral nucleic acids inside the capsid. However, the relevance of capsid pressure to viral infection has not...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2019-08-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/47212 |
| id |
doaj-3d12f94a8e8341b29bf84ba9f9434a75 |
|---|---|
| record_format |
Article |
| spelling |
doaj-3d12f94a8e8341b29bf84ba9f9434a752021-05-05T17:49:33ZengeLife Sciences Publications LtdeLife2050-084X2019-08-01810.7554/eLife.47212Pressure-driven release of viral genome into a host nucleus is a mechanism leading to herpes infectionAlberto Brandariz-Nuñez0Ting Liu1Te Du2Alex Evilevitch3https://orcid.org/0000-0002-0245-9574Department of Pathobiology, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, Urbana, United StatesDepartment of Physics, Carnegie Mellon University, Pittsburgh, United StatesThe Marjorie B. Kovler Viral Oncology Laboratories, The University of Chicago, Chicago, United StatesDepartment of Pathobiology, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, Urbana, United States; Department of Experimental Medical Sciences, Lund University, Lund, SwedenMany viruses previously have been shown to have pressurized genomes inside their viral protein shell, termed the capsid. This pressure results from the tight confinement of negatively charged viral nucleic acids inside the capsid. However, the relevance of capsid pressure to viral infection has not been demonstrated. In this work, we show that the internal DNA pressure of tens of atmospheres inside a herpesvirus capsid powers ejection of the viral genome into a host cell nucleus. To our knowledge, this provides the first demonstration of a pressure-dependent mechanism of viral genome penetration into a host nucleus, leading to infection of eukaryotic cells.https://elifesciences.org/articles/47212herpesvirusDNA pressurecell nucleusherpes capsidDNA ejectionpressure-driven DNA ejection |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Alberto Brandariz-Nuñez Ting Liu Te Du Alex Evilevitch |
| spellingShingle |
Alberto Brandariz-Nuñez Ting Liu Te Du Alex Evilevitch Pressure-driven release of viral genome into a host nucleus is a mechanism leading to herpes infection eLife herpesvirus DNA pressure cell nucleus herpes capsid DNA ejection pressure-driven DNA ejection |
| author_facet |
Alberto Brandariz-Nuñez Ting Liu Te Du Alex Evilevitch |
| author_sort |
Alberto Brandariz-Nuñez |
| title |
Pressure-driven release of viral genome into a host nucleus is a mechanism leading to herpes infection |
| title_short |
Pressure-driven release of viral genome into a host nucleus is a mechanism leading to herpes infection |
| title_full |
Pressure-driven release of viral genome into a host nucleus is a mechanism leading to herpes infection |
| title_fullStr |
Pressure-driven release of viral genome into a host nucleus is a mechanism leading to herpes infection |
| title_full_unstemmed |
Pressure-driven release of viral genome into a host nucleus is a mechanism leading to herpes infection |
| title_sort |
pressure-driven release of viral genome into a host nucleus is a mechanism leading to herpes infection |
| publisher |
eLife Sciences Publications Ltd |
| series |
eLife |
| issn |
2050-084X |
| publishDate |
2019-08-01 |
| description |
Many viruses previously have been shown to have pressurized genomes inside their viral protein shell, termed the capsid. This pressure results from the tight confinement of negatively charged viral nucleic acids inside the capsid. However, the relevance of capsid pressure to viral infection has not been demonstrated. In this work, we show that the internal DNA pressure of tens of atmospheres inside a herpesvirus capsid powers ejection of the viral genome into a host cell nucleus. To our knowledge, this provides the first demonstration of a pressure-dependent mechanism of viral genome penetration into a host nucleus, leading to infection of eukaryotic cells. |
| topic |
herpesvirus DNA pressure cell nucleus herpes capsid DNA ejection pressure-driven DNA ejection |
| url |
https://elifesciences.org/articles/47212 |
| work_keys_str_mv |
AT albertobrandariznunez pressuredrivenreleaseofviralgenomeintoahostnucleusisamechanismleadingtoherpesinfection AT tingliu pressuredrivenreleaseofviralgenomeintoahostnucleusisamechanismleadingtoherpesinfection AT tedu pressuredrivenreleaseofviralgenomeintoahostnucleusisamechanismleadingtoherpesinfection AT alexevilevitch pressuredrivenreleaseofviralgenomeintoahostnucleusisamechanismleadingtoherpesinfection |
| _version_ |
1721458964161363968 |