<b>Enzyme expression in <i>indica</i> and <i>japonica</i> rice cultivars under saline stress</b> - doi: 10.4025/actascibiolsci.v34i4.8535

• Main Authors: Luciano do Amarante, Geri Eduardo Meneghello, Cristina Rodrigues Mendes, Paulo Dejalma Zimmer, Nei Fernandes Lopes, Maria da Graça de Souza Lima
• Format: Article
• Language: English
• Published: Universidade Estadual de Maringá 2012-09-01
• Series: Acta Scientiarum : Biological Sciences
• Subjects: electrophoresis; isoenzyme; krebs cycle; O. sativa L.; salinity
• Online Access: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/8535

<p class="aTitulodoArtigo">The southern State of Rio Grande do Sul (RS) is the main rice producer in Brazil with a 60% participation of the national production and 86% participation of the region. Rice culture irrigation system is done by flooding, which leads to soil salinization, a major environmental constraint to production since it alters the plants’ metabolism exposed to this type of stress. The <em>indica</em> cultivar, widely used in RS, has a higher sensitivity to salinity when compared to that of the <em>japonica</em> cultivar in other physiological aspects. Current research analyzes enzymes expression involved in salt-subjected <em>indica</em> and <em>japonica</em> rice cultivars’ respiration. <em>Oryza sativa</em> L. spp. <em>japonica </em>S.Kato (BRS Bojuru, IAS 12-9 Formosa and Goyakuman) and <em>Oryza sativa</em> L. spp. <em>indica</em> S. Kato (BRS Taim-7, BRS Atalanta and BRS Querencia) were the cultivars employed. Seedlings were transferred to 15 L basins containing 50% Hoagland nutrient solution increased by 0, 25, 50, 75 and 100 mM NaCl, and collected at 14, 28 and 42 days after transfer (DAT). Plant tissues were macerated and placed in <em>eppendorf</em> tubes with Scandálios extractor solution. Electrophoresis was performed in 7% of the polyacrylamide gels in vertical vats. Bands were revealed for the following enzymes systems: esterase, alcohol dehydrogenase, phosphoglucoisomerase, malate dehydrogenase, malic enzyme and alpha amylase. The enzymes expression was greater in subspecies <em>japonica,</em> with more intense bands in proportion to salinity increase. Results show that enzyme systems are involved in the salinity defense mechanisms in <em>O. sativa</em> spp. <em>japonica </em>cultivar.</p> <p class="akeyword"> </p><br />