PCR effects of melting temperature adjustment of individual primers in degenerate primer pools

PCR effects of melting temperature adjustment of individual primers in degenerate primer pools

Deep sequencing of small subunit ribosomal RNA (SSU rRNA) gene amplicons continues to be the most common approach for characterization of complex microbial communities. PCR amplifications of conserved regions of SSU rRNA genes often employ degenerate pools of primers to enable targeting of a broad s...

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Bibliographic Details
Main Authors: Ankur Naqib, Trisha Jeon, Kevin Kunstman, Weihua Wang, Yiding Shen, Dagmar Sweeney, Marieta Hyde, Stefan J. Green
Format: Article
Language:English
Published: PeerJ Inc. 2019-03-01
Series:PeerJ
Subjects:
Microbiome
phiX
Degenerate primers
NGS
Primer design
16S rRNA
Online Access:https://peerj.com/articles/6570.pdf

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https://peerj.com/articles/6570.pdf

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