Genetically Engineered Phage Induced Selective H9c2 Cardiomyocytes Patterning in PDMS Microgrooves

• Main Authors: Youngjun Kim, Chunga Kwon, Hojeong Jeon
• Format: Article
• Language: English
• Published: MDPI AG 2017-08-01
• Series: Materials
• Subjects: polydimethylsiloxane (PDMS); micro-patterns; RGD-phage; cell-surface interaction; rat H9c2 cardiomyocytes
• Online Access: https://www.mdpi.com/1996-1944/10/8/973

A micro-patterned cell adhesive surface was prepared for future design of medical devices. One-dimensional polydimethylsiloxane (PDMS) micro-patterns were prepared by a photolithography process. Afterwards, recombinant filamentous phages that displayed a short binding motif with a cell adhesive peptide (-RGD-) on p8 proteins were immobilized on PDMS microgrooves through simple contact printing to study the cellular response of rat H9c2 cardiomyocyte. While the cell density decreased on PDMS micro-patterns, we observed enhanced cell proliferation and cell to surface interaction on the RGD-phage coated PDMS microgrooves. The RGD-phage coating also supported a better alignment of cell spreading rather than isotropic cell growths as we observed on non-pattered PDMS surface.