Rescuing eGFP-Tagged Canine Distemper Virus for 40 Serial Passages Separately in Ribavirin- and Non-Treated Cells: Comparative Analysis of Viral Mutation Profiles

Rescuing eGFP-Tagged Canine Distemper Virus for 40 Serial Passages Separately in Ribavirin- and Non-Treated Cells: Comparative Analysis of Viral Mutation Profiles

Due to lacking a proofreading mechanism in their RNA-dependent RNA polymerases (RdRp), RNA viruses generally possess high mutation frequencies, making them evolve rapidly to form viral quasispecies during serial passages in cells, especially treated with mutagens, like ribavirin. Canine distemper vi...

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Bibliographic Details
Main Authors: Fuxiao Liu, Ning Wang, Jiahui Lin, Qianqian Wang, Yilan Huang, Youming Zhang, Hu Shan
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-09-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
rCDV-eGFP
ribavirin
quasispecies
next-generation sequencing
SNM
high-fidelity
Online Access:https://www.frontiersin.org/articles/10.3389/fcimb.2021.746926/full

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