Sensitive commercial NASBA assay for the detection of respiratory syncytial virus in clinical specimen.

Sensitive commercial NASBA assay for the detection of respiratory syncytial virus in clinical specimen.

The aim of the study was to evaluate the usability of three diagnostic procedures for the detection of respiratory syncytial virus in clinical samples. Therefore, the FDA cleared CE marked NOW(R) RSV ELISA, the NucliSENS EasyQ RSV A+B NASBA, and a literature based inhouse RT-PCR protocol were compar...

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Main Authors: Ramona Liza Tillmann, Arne Simon, Andreas Müller, Oliver Schildgen
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2007-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2137935?pdf=render
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spelling doaj-3c76e009388b464fad74addeac5c57592020-11-25T02:27:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032007-01-01212e135710.1371/journal.pone.0001357Sensitive commercial NASBA assay for the detection of respiratory syncytial virus in clinical specimen.Ramona Liza TillmannArne SimonAndreas MüllerOliver SchildgenThe aim of the study was to evaluate the usability of three diagnostic procedures for the detection of respiratory syncytial virus in clinical samples. Therefore, the FDA cleared CE marked NOW(R) RSV ELISA, the NucliSENS EasyQ RSV A+B NASBA, and a literature based inhouse RT-PCR protocol were compared for their relative sensitivities. Thereby, NASBA turned out to be the most sensitive method with a total number of 80 RSV positive samples out of a cohort of 251 nasopharyngeal washings from patients suffering from clinical symptoms, followed by the inhouse RT-PCR (62/251) and ELISA (52/251). Thus, NASBA may serve as a rapid and highly sensitive alternative for RSV diagnostics.http://europepmc.org/articles/PMC2137935?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Ramona Liza Tillmann
Arne Simon
Andreas Müller
Oliver Schildgen
spellingShingle Ramona Liza Tillmann
Arne Simon
Andreas Müller
Oliver Schildgen
Sensitive commercial NASBA assay for the detection of respiratory syncytial virus in clinical specimen.
PLoS ONE
author_facet Ramona Liza Tillmann
Arne Simon
Andreas Müller
Oliver Schildgen
author_sort Ramona Liza Tillmann
title Sensitive commercial NASBA assay for the detection of respiratory syncytial virus in clinical specimen.
title_short Sensitive commercial NASBA assay for the detection of respiratory syncytial virus in clinical specimen.
title_full Sensitive commercial NASBA assay for the detection of respiratory syncytial virus in clinical specimen.
title_fullStr Sensitive commercial NASBA assay for the detection of respiratory syncytial virus in clinical specimen.
title_full_unstemmed Sensitive commercial NASBA assay for the detection of respiratory syncytial virus in clinical specimen.
title_sort sensitive commercial nasba assay for the detection of respiratory syncytial virus in clinical specimen.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2007-01-01
description The aim of the study was to evaluate the usability of three diagnostic procedures for the detection of respiratory syncytial virus in clinical samples. Therefore, the FDA cleared CE marked NOW(R) RSV ELISA, the NucliSENS EasyQ RSV A+B NASBA, and a literature based inhouse RT-PCR protocol were compared for their relative sensitivities. Thereby, NASBA turned out to be the most sensitive method with a total number of 80 RSV positive samples out of a cohort of 251 nasopharyngeal washings from patients suffering from clinical symptoms, followed by the inhouse RT-PCR (62/251) and ELISA (52/251). Thus, NASBA may serve as a rapid and highly sensitive alternative for RSV diagnostics.
url http://europepmc.org/articles/PMC2137935?pdf=render
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AT arnesimon sensitivecommercialnasbaassayforthedetectionofrespiratorysyncytialvirusinclinicalspecimen
AT andreasmuller sensitivecommercialnasbaassayforthedetectionofrespiratorysyncytialvirusinclinicalspecimen
AT oliverschildgen sensitivecommercialnasbaassayforthedetectionofrespiratorysyncytialvirusinclinicalspecimen
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