Transmembrane but not soluble helices fold inside the ribosome tunnel

Integral membrane proteins are assembled into the ER membrane via the ribosome-translocon channel. Here authors use in vitro translation assays and MD simulations to show that folding in the ribosome is favorable for TM helices, but unfavorable for soluble helices.

Bibliographic Details
Main Authors:	Manuel Bañó-Polo, Carlos Baeza-Delgado, Silvia Tamborero, Anthony Hazel, Brayan Grau, IngMarie Nilsson, Paul Whitley, James C. Gumbart, Gunnar von Heijne, Ismael Mingarro
Format:	Article
Language:	English
Published:	Nature Publishing Group 2018-12-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-018-07554-7

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spelling	doaj-3c5b89887b174ab79e5d3eb70d8006cc2021-05-11T09:37:03ZengNature Publishing GroupNature Communications2041-17232018-12-01911910.1038/s41467-018-07554-7Transmembrane but not soluble helices fold inside the ribosome tunnelManuel Bañó-Polo0Carlos Baeza-Delgado1Silvia Tamborero2Anthony Hazel3Brayan Grau4IngMarie Nilsson5Paul Whitley6James C. Gumbart7Gunnar von Heijne8Ismael Mingarro9Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (ERI BioTecMed), Departament de Bioquímica i Biologia Molecular, Universitat de ValènciaEstructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (ERI BioTecMed), Departament de Bioquímica i Biologia Molecular, Universitat de ValènciaEstructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (ERI BioTecMed), Departament de Bioquímica i Biologia Molecular, Universitat de ValènciaSchool of Physics, School of Chemistry and Biochemistry, Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of TechnologyEstructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (ERI BioTecMed), Departament de Bioquímica i Biologia Molecular, Universitat de ValènciaCenter for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm UniversityDepartment of Biology and Biochemistry, Centre for Regenerative Medicine, University of BathSchool of Physics, School of Chemistry and Biochemistry, Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of TechnologyCenter for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm UniversityEstructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (ERI BioTecMed), Departament de Bioquímica i Biologia Molecular, Universitat de ValènciaIntegral membrane proteins are assembled into the ER membrane via the ribosome-translocon channel. Here authors use in vitro translation assays and MD simulations to show that folding in the ribosome is favorable for TM helices, but unfavorable for soluble helices.https://doi.org/10.1038/s41467-018-07554-7
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Manuel Bañó-Polo Carlos Baeza-Delgado Silvia Tamborero Anthony Hazel Brayan Grau IngMarie Nilsson Paul Whitley James C. Gumbart Gunnar von Heijne Ismael Mingarro
spellingShingle	Manuel Bañó-Polo Carlos Baeza-Delgado Silvia Tamborero Anthony Hazel Brayan Grau IngMarie Nilsson Paul Whitley James C. Gumbart Gunnar von Heijne Ismael Mingarro Transmembrane but not soluble helices fold inside the ribosome tunnel Nature Communications
author_facet	Manuel Bañó-Polo Carlos Baeza-Delgado Silvia Tamborero Anthony Hazel Brayan Grau IngMarie Nilsson Paul Whitley James C. Gumbart Gunnar von Heijne Ismael Mingarro
author_sort	Manuel Bañó-Polo
title	Transmembrane but not soluble helices fold inside the ribosome tunnel
title_short	Transmembrane but not soluble helices fold inside the ribosome tunnel
title_full	Transmembrane but not soluble helices fold inside the ribosome tunnel
title_fullStr	Transmembrane but not soluble helices fold inside the ribosome tunnel
title_full_unstemmed	Transmembrane but not soluble helices fold inside the ribosome tunnel
title_sort	transmembrane but not soluble helices fold inside the ribosome tunnel
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2018-12-01
description	Integral membrane proteins are assembled into the ER membrane via the ribosome-translocon channel. Here authors use in vitro translation assays and MD simulations to show that folding in the ribosome is favorable for TM helices, but unfavorable for soluble helices.
url	https://doi.org/10.1038/s41467-018-07554-7
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Transmembrane but not soluble helices fold inside the ribosome tunnel

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