Mitochondria-specific delivery system for targeted regulation of mitochondrial gene expression

• Main Authors: Shicheng Su, Shuwen Cao, Jiayu Liu, Xiaoding Xu
• Format: Article
• Language: English
• Published: Elsevier 2021-03-01
• Series: STAR Protocols
• Subjects: Genetics; Molecular Biology; Gene Expression
• Online Access: http://www.sciencedirect.com/science/article/pii/S2666166720302628

Summary: Targeted regulation of mitochondrial gene expression is challenging due to the lack of a mitochondria-specific delivery system. We have previously developed various stimuli-responsive nanoparticle (NP)-based delivery systems to transport nucleic acids for regulation of target gene expression. This protocol describes the design and preparation of an NP platform for mitochondria-specific gene delivery (mito-NP). We use mito-NP in primary liver fibroblasts that are transplanted into mice. Mito-NP can be used to deliver various nucleic acid therapeutics and to treat mitochondria-regulated diseases.For complete details on the use and execution of this protocol, please refer to Zhao et al. (2020).