Production, purification, sequencing and activity spectra of mutacins D-123.1 and F-59.1

• Main Authors: LaPointe Gisèle, Nicolas Guillaume G, Lavoie Marc C
• Format: Article
• Language: English
• Published: BMC 2011-04-01
• Series: BMC Microbiology
• Subjects: bacteriocin; lantibiotic; mutacin; pediocin; <it>Streptococcus mutans</it>
• Online Access: http://www.biomedcentral.com/1471-2180/11/69
• ISSN: 1471-2180

<p>Abstract</p> <p>Background</p> <p>The increase in bacterial resistance to antibiotics impels the development of new anti-bacterial substances. Mutacins (bacteriocins) are small antibacterial peptides produced by <it>Streptococcus mutans </it>showing activity against bacterial pathogens. The objective of the study was to produce and characterise additional mutacins in order to find new useful antibacterial substances.</p> <p>Results</p> <p>Mutacin F-59.1 was produced in liquid media by <it>S. mutans </it>59.1 while production of mutacin D-123.1 by <it>S. mutans </it>123.1 was obtained in semi-solid media. Mutacins were purified by hydrophobic chromatography. The amino acid sequences of the mutacins were obtained by Edman degradation and their molecular mass was determined by mass spectrometry. Mutacin F-59.1 consists of 25 amino acids, containing the YGNGV consensus sequence of pediocin-like bacteriocins with a molecular mass calculated at 2719 Da. Mutacin D-123.1 has an identical molecular mass (2364 Da) with the same first 9 amino acids as mutacin I. Mutacins D-123.1 and F-59.1 have wide activity spectra inhibiting human and food-borne pathogens. The lantibiotic mutacin D-123.1 possesses a broader activity spectrum than mutacin F-59.1 against the bacterial strains tested.</p> <p>Conclusion</p> <p>Mutacin F-59.1 is the first pediocin-like bacteriocin identified and characterised that is produced by <it>Streptococcus mutans</it>. Mutacin D-123.1 appears to be identical to mutacin I previously identified in different strains of <it>S. mutans</it>.</p>