<i>Yersinia pseudotuberculosis </i>Typing Using Multi-Locus Variable-Number Tandem Repeat Analysis

<i>Yersinia pseudotuberculosis </i>Typing Using Multi-Locus Variable-Number Tandem Repeat Analysis

Estimated has been efficacy of MLVA25 method, used for differentiation and clusterization of Yersinia pestis strains, in reference to Yersinia pseudotuberculosis strains, as well as the search of Y. pseudotuberculosis strains mostly closely related to Y. pestis . Applied was the reduced version of t...

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Main Authors: V. V. Evseeva, M. E. Platonov, S. V. Dentovskaya, A. P. Anisimov
Format: Article
Language:Russian
Published: Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” 2015-12-01
Series:Проблемы особо опасных инфекций
Subjects:
yersinia pseudotuberculosis
vntr
mlva
генотипирование
genotyping
Online Access:https://journal.microbe.ru/jour/article/view/266
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spelling doaj-3c09f3f44efb4b23bb86ef07e5f341c22021-07-22T07:51:38ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2015-12-0104555710.21055/0370-1069-2015-4-55-57266<i>Yersinia pseudotuberculosis </i>Typing Using Multi-Locus Variable-Number Tandem Repeat AnalysisV. V. Evseeva0M. E. Platonov1S. V. Dentovskaya2A. P. Anisimov3State Research Center for Applied Microbiology and BiotechnologyState Research Center for Applied Microbiology and BiotechnologyState Research Center for Applied Microbiology and BiotechnologyState Research Center for Applied Microbiology and BiotechnologyEstimated has been efficacy of MLVA25 method, used for differentiation and clusterization of Yersinia pestis strains, in reference to Yersinia pseudotuberculosis strains, as well as the search of Y. pseudotuberculosis strains mostly closely related to Y. pestis . Applied was the reduced version of the technique deploying 14 out of 25 VNTR loci. Comparative study of 71 Y. pseudotuberculosis isolates and five Y. pestis strains by means of MLVA25 and MLVA14 variants revealed 75 and 54 genotypes, respectively. Y. pseudotuberculosis strains of certain serovars corresponded to certain clusters of MLVA types. According to MLVA14 typing of 221 Y. pseudotuberculosis isolates the clusters including the strains belonging to MLST types ST19 (serovar O:3) and ST43 (serovar O:1b) were the most closely related to Y. pestis .https://journal.microbe.ru/jour/article/view/266yersinia pseudotuberculosisvntrmlvaгенотипированиеgenotyping
collection DOAJ
language Russian
format Article
sources DOAJ
author V. V. Evseeva
M. E. Platonov
S. V. Dentovskaya
A. P. Anisimov
spellingShingle V. V. Evseeva
M. E. Platonov
S. V. Dentovskaya
A. P. Anisimov
<i>Yersinia pseudotuberculosis </i>Typing Using Multi-Locus Variable-Number Tandem Repeat Analysis
Проблемы особо опасных инфекций
yersinia pseudotuberculosis
vntr
mlva
генотипирование
genotyping
author_facet V. V. Evseeva
M. E. Platonov
S. V. Dentovskaya
A. P. Anisimov
author_sort V. V. Evseeva
title <i>Yersinia pseudotuberculosis </i>Typing Using Multi-Locus Variable-Number Tandem Repeat Analysis
title_short <i>Yersinia pseudotuberculosis </i>Typing Using Multi-Locus Variable-Number Tandem Repeat Analysis
title_full <i>Yersinia pseudotuberculosis </i>Typing Using Multi-Locus Variable-Number Tandem Repeat Analysis
title_fullStr <i>Yersinia pseudotuberculosis </i>Typing Using Multi-Locus Variable-Number Tandem Repeat Analysis
title_full_unstemmed <i>Yersinia pseudotuberculosis </i>Typing Using Multi-Locus Variable-Number Tandem Repeat Analysis
title_sort <i>yersinia pseudotuberculosis </i>typing using multi-locus variable-number tandem repeat analysis
publisher Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
series Проблемы особо опасных инфекций
issn 0370-1069
2658-719X
publishDate 2015-12-01
description Estimated has been efficacy of MLVA25 method, used for differentiation and clusterization of Yersinia pestis strains, in reference to Yersinia pseudotuberculosis strains, as well as the search of Y. pseudotuberculosis strains mostly closely related to Y. pestis . Applied was the reduced version of the technique deploying 14 out of 25 VNTR loci. Comparative study of 71 Y. pseudotuberculosis isolates and five Y. pestis strains by means of MLVA25 and MLVA14 variants revealed 75 and 54 genotypes, respectively. Y. pseudotuberculosis strains of certain serovars corresponded to certain clusters of MLVA types. According to MLVA14 typing of 221 Y. pseudotuberculosis isolates the clusters including the strains belonging to MLST types ST19 (serovar O:3) and ST43 (serovar O:1b) were the most closely related to Y. pestis .
topic yersinia pseudotuberculosis
vntr
mlva
генотипирование
genotyping
url https://journal.microbe.ru/jour/article/view/266
work_keys_str_mv AT vvevseeva iyersiniapseudotuberculosisitypingusingmultilocusvariablenumbertandemrepeatanalysis
AT meplatonov iyersiniapseudotuberculosisitypingusingmultilocusvariablenumbertandemrepeatanalysis
AT svdentovskaya iyersiniapseudotuberculosisitypingusingmultilocusvariablenumbertandemrepeatanalysis
AT apanisimov iyersiniapseudotuberculosisitypingusingmultilocusvariablenumbertandemrepeatanalysis
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