Preparation of apoE-free rat low density lipoprotein for catabolic studies.

Preparation of apoE-free rat low density lipoprotein for catabolic studies.

The use of serum from rat fetuses instead of serum from adult rats for preparation of LDL by preparative ultracentrifugation leads to an LDL fraction containing apoB-100 and apoB-95 as the only protein moieties without need for any further purification. The yield of LDL is five times greater compare...

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Main Authors: B Schlag, L Winkler, D Plonné, U Dürer, R Dargel
Format: Article
Language:English
Published: Elsevier 1987-12-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520385850
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spelling doaj-3c01dc3e380c4e33b530267763eeed4a2021-04-25T04:19:59ZengElsevierJournal of Lipid Research0022-22751987-12-01281215211524Preparation of apoE-free rat low density lipoprotein for catabolic studies.B Schlag0L Winkler1D Plonné2U Dürer3R Dargel4Institute of Pathological Biochemistry, Friedrich Schiller University, Jena, GDR.Institute of Pathological Biochemistry, Friedrich Schiller University, Jena, GDR.Institute of Pathological Biochemistry, Friedrich Schiller University, Jena, GDR.Institute of Pathological Biochemistry, Friedrich Schiller University, Jena, GDR.Institute of Pathological Biochemistry, Friedrich Schiller University, Jena, GDR.The use of serum from rat fetuses instead of serum from adult rats for preparation of LDL by preparative ultracentrifugation leads to an LDL fraction containing apoB-100 and apoB-95 as the only protein moieties without need for any further purification. The yield of LDL is five times greater compared to the use of adult rat serum. Lipid composition and particle size of LDL from fetal and adult rats are quite similar. The method described allows a simple way for preparation of sufficient amounts of apoE-free LDL for use in metabolic studies.http://www.sciencedirect.com/science/article/pii/S0022227520385850
collection DOAJ
language English
format Article
sources DOAJ
author B Schlag
L Winkler
D Plonné
U Dürer
R Dargel
spellingShingle B Schlag
L Winkler
D Plonné
U Dürer
R Dargel
Preparation of apoE-free rat low density lipoprotein for catabolic studies.
Journal of Lipid Research
author_facet B Schlag
L Winkler
D Plonné
U Dürer
R Dargel
author_sort B Schlag
title Preparation of apoE-free rat low density lipoprotein for catabolic studies.
title_short Preparation of apoE-free rat low density lipoprotein for catabolic studies.
title_full Preparation of apoE-free rat low density lipoprotein for catabolic studies.
title_fullStr Preparation of apoE-free rat low density lipoprotein for catabolic studies.
title_full_unstemmed Preparation of apoE-free rat low density lipoprotein for catabolic studies.
title_sort preparation of apoe-free rat low density lipoprotein for catabolic studies.
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1987-12-01
description The use of serum from rat fetuses instead of serum from adult rats for preparation of LDL by preparative ultracentrifugation leads to an LDL fraction containing apoB-100 and apoB-95 as the only protein moieties without need for any further purification. The yield of LDL is five times greater compared to the use of adult rat serum. Lipid composition and particle size of LDL from fetal and adult rats are quite similar. The method described allows a simple way for preparation of sufficient amounts of apoE-free LDL for use in metabolic studies.
url http://www.sciencedirect.com/science/article/pii/S0022227520385850
work_keys_str_mv AT bschlag preparationofapoefreeratlowdensitylipoproteinforcatabolicstudies
AT lwinkler preparationofapoefreeratlowdensitylipoproteinforcatabolicstudies
AT dplonne preparationofapoefreeratlowdensitylipoproteinforcatabolicstudies
AT udurer preparationofapoefreeratlowdensitylipoproteinforcatabolicstudies
AT rdargel preparationofapoefreeratlowdensitylipoproteinforcatabolicstudies
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