Isolation and culture of protoplast from leaves of Lactuca sativa
Protoplasts were isolated from leaves of lettuce (Lactuca sativa L.) seedlings after in vitro germination for 25, 30, 40 and 50 days. The leaves were stripped and incubated in various combinations of cellulase and pectinase. Protoplasts were cultured on MS medium containing various kinds and concent...
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2007-07-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
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| Online Access: | http://www.sjst.psu.ac.th/journal/29-4_online/0125-3395-29-4-0929-0935.pdf |