Sea urchin (Strongylocentrotus intermedius) polysaccharide enhanced BMP-2 induced osteogenic differentiation and its structural analysis

Sea urchin (Strongylocentrotus intermedius) polysaccharide enhanced BMP-2 induced osteogenic differentiation and its structural analysis

A new sea urchin polysaccharide (SUP) was extracted from the gonad of sea urchin (Strongylocentrotus intermedius), and its bioactivity to synergistically enhance the functionality of bone morphogenetic protein-2 (BMP-2) induced osteoblastic differentiation was characterized. Results showed that the...

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Main Authors: Jingfeng Yang, Meng Yi, Jinfeng Pan, Jun Zhao, Liming Sun, Xinping Lin, Yuegang Cao, Lu Huang, Beiwei Zhu, Chenxu Yu
Format: Article
Language:English
Published: Elsevier 2015-04-01
Series:Journal of Functional Foods
Subjects:
Bone
Regeneration
Alkaline phosphatase
C2C12
Online Access:http://www.sciencedirect.com/science/article/pii/S1756464615000870
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spelling doaj-3bdd74f110144ad299c110635545a41f2021-04-29T04:43:57ZengElsevierJournal of Functional Foods1756-46462015-04-0114519528Sea urchin (Strongylocentrotus intermedius) polysaccharide enhanced BMP-2 induced osteogenic differentiation and its structural analysisJingfeng Yang0Meng Yi1Jinfeng Pan2Jun Zhao3Liming Sun4Xinping Lin5Yuegang Cao6Lu Huang7Beiwei Zhu8Chenxu Yu9School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, China; Corresponding author. School of Food Science and Technology, Dalian Polytechnic University, No.1 Qinggongyuan, Ganjingzi district, Dalian 116034, China. Tel.: +86 411 86323262; fax: +86 411 86323262.School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, ChinaDepartment of Agricultural and Biosystems Engineering, Iowa State University, IA 50011, USAA new sea urchin polysaccharide (SUP) was extracted from the gonad of sea urchin (Strongylocentrotus intermedius), and its bioactivity to synergistically enhance the functionality of bone morphogenetic protein-2 (BMP-2) induced osteoblastic differentiation was characterized. Results showed that the SUP is homogenous with an average molecular weight of 4.4 kDa, and it is mainly composed of mannose with a small number of glucose and N-acetylglucosamine, with a backbone of alternating 1, 4-D-Man and 1, 3-D-Man residues. The SUP was shown to synergistically enhance BMP-2 induced osteoblastic differentiation in C2C12 myoblast cells, evidenced by a significant increase in the activity of alkaline phosphatase (ALP), when the concentration of the SUP was higher than 10 µg/mL. The elevated ALP activity was also confirmed by histochemical staining of C2C12 myoblast cells. These results suggest that the SUP can promote bone regeneration and may serve as a functional food supplement for bone health.http://www.sciencedirect.com/science/article/pii/S1756464615000870BoneRegenerationAlkaline phosphataseC2C12
collection DOAJ
language English
format Article
sources DOAJ
author Jingfeng Yang
Meng Yi
Jinfeng Pan
Jun Zhao
Liming Sun
Xinping Lin
Yuegang Cao
Lu Huang
Beiwei Zhu
Chenxu Yu
spellingShingle Jingfeng Yang
Meng Yi
Jinfeng Pan
Jun Zhao
Liming Sun
Xinping Lin
Yuegang Cao
Lu Huang
Beiwei Zhu
Chenxu Yu
Sea urchin (Strongylocentrotus intermedius) polysaccharide enhanced BMP-2 induced osteogenic differentiation and its structural analysis
Journal of Functional Foods
Bone
Regeneration
Alkaline phosphatase
C2C12
author_facet Jingfeng Yang
Meng Yi
Jinfeng Pan
Jun Zhao
Liming Sun
Xinping Lin
Yuegang Cao
Lu Huang
Beiwei Zhu
Chenxu Yu
author_sort Jingfeng Yang
title Sea urchin (Strongylocentrotus intermedius) polysaccharide enhanced BMP-2 induced osteogenic differentiation and its structural analysis
title_short Sea urchin (Strongylocentrotus intermedius) polysaccharide enhanced BMP-2 induced osteogenic differentiation and its structural analysis
title_full Sea urchin (Strongylocentrotus intermedius) polysaccharide enhanced BMP-2 induced osteogenic differentiation and its structural analysis
title_fullStr Sea urchin (Strongylocentrotus intermedius) polysaccharide enhanced BMP-2 induced osteogenic differentiation and its structural analysis
title_full_unstemmed Sea urchin (Strongylocentrotus intermedius) polysaccharide enhanced BMP-2 induced osteogenic differentiation and its structural analysis
title_sort sea urchin (strongylocentrotus intermedius) polysaccharide enhanced bmp-2 induced osteogenic differentiation and its structural analysis
publisher Elsevier
series Journal of Functional Foods
issn 1756-4646
publishDate 2015-04-01
description A new sea urchin polysaccharide (SUP) was extracted from the gonad of sea urchin (Strongylocentrotus intermedius), and its bioactivity to synergistically enhance the functionality of bone morphogenetic protein-2 (BMP-2) induced osteoblastic differentiation was characterized. Results showed that the SUP is homogenous with an average molecular weight of 4.4 kDa, and it is mainly composed of mannose with a small number of glucose and N-acetylglucosamine, with a backbone of alternating 1, 4-D-Man and 1, 3-D-Man residues. The SUP was shown to synergistically enhance BMP-2 induced osteoblastic differentiation in C2C12 myoblast cells, evidenced by a significant increase in the activity of alkaline phosphatase (ALP), when the concentration of the SUP was higher than 10 µg/mL. The elevated ALP activity was also confirmed by histochemical staining of C2C12 myoblast cells. These results suggest that the SUP can promote bone regeneration and may serve as a functional food supplement for bone health.
topic Bone
Regeneration
Alkaline phosphatase
C2C12
url http://www.sciencedirect.com/science/article/pii/S1756464615000870
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