An Ultrasensitive Gold Nanoparticle-based Lateral Flow Test for the Detection of Active Botulinum Neurotoxin Type A

Abstract Botulism is a severe and potentially lethal paralytic disease caused by several botulinum neurotoxin-producing Clostridia spp. In China, the majority of the cases caused by botulism were from less-developed rural areas. Here, we designed specific substrate peptides and reconfigured gold nan...

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Main Authors: Jing Liu, Shan Gao, Lin Kang, Bin Ji, Wenwen Xin, Jingjing Kang, Ping Li, Jie Gao, Hanbin Wang, Jinglin Wang, Hao Yang
Format: Article
Language:English
Published: SpringerOpen 2017-03-01
Series:Nanoscale Research Letters
Subjects:
Online Access:http://link.springer.com/article/10.1186/s11671-017-1944-9
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spelling doaj-3ba424192da241f4ac85fdfdab08cfb12020-11-25T00:34:38ZengSpringerOpenNanoscale Research Letters1931-75731556-276X2017-03-011211610.1186/s11671-017-1944-9An Ultrasensitive Gold Nanoparticle-based Lateral Flow Test for the Detection of Active Botulinum Neurotoxin Type AJing Liu0Shan Gao1Lin Kang2Bin Ji3Wenwen Xin4Jingjing Kang5Ping Li6Jie Gao7Hanbin Wang8Jinglin Wang9Hao Yang10State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and EpidemiologyState Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and EpidemiologyState Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and EpidemiologyState Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and EpidemiologyState Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and EpidemiologyState Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and EpidemiologyState Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and EpidemiologyState Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and EpidemiologyThe 307th Hospital of Military Chinese People’s Liberation ArmyState Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and EpidemiologyState Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and EpidemiologyAbstract Botulism is a severe and potentially lethal paralytic disease caused by several botulinum neurotoxin-producing Clostridia spp. In China, the majority of the cases caused by botulism were from less-developed rural areas. Here, we designed specific substrate peptides and reconfigured gold nanoparticle-based lateral flow test strip (LFTS) to develop an endopeptidase-based lateral flow assay for the diagnosis of botulism. We performed this lateral flow assay on botulinum neurotoxin-spiked human serum samples. The as-prepared LFTS had excellent performance in the detection of botulinum neurotoxin using only 1 μL of simulated serum, and its sensitivity and specificity were comparable to that of mouse lethality assay. Moreover, the assay takes only half a day and does not require highly trained laboratory staff, specialized facility, or equipment. Finally, our LFTS can be potentially extended to other serotypes of BoNTs by designing specific substrate peptides against the different types of BoNTs. Overall, we demonstrate a strategy by which LFTS and endopeptidase activity assays can be integrated to achieve facile and economic diagnosis of botulism in resource-limited settings.http://link.springer.com/article/10.1186/s11671-017-1944-9BotulismBotulinum toxinLateral flow assayEndopeptidase activity assayMouse lethality assay
collection DOAJ
language English
format Article
sources DOAJ
author Jing Liu
Shan Gao
Lin Kang
Bin Ji
Wenwen Xin
Jingjing Kang
Ping Li
Jie Gao
Hanbin Wang
Jinglin Wang
Hao Yang
spellingShingle Jing Liu
Shan Gao
Lin Kang
Bin Ji
Wenwen Xin
Jingjing Kang
Ping Li
Jie Gao
Hanbin Wang
Jinglin Wang
Hao Yang
An Ultrasensitive Gold Nanoparticle-based Lateral Flow Test for the Detection of Active Botulinum Neurotoxin Type A
Nanoscale Research Letters
Botulism
Botulinum toxin
Lateral flow assay
Endopeptidase activity assay
Mouse lethality assay
author_facet Jing Liu
Shan Gao
Lin Kang
Bin Ji
Wenwen Xin
Jingjing Kang
Ping Li
Jie Gao
Hanbin Wang
Jinglin Wang
Hao Yang
author_sort Jing Liu
title An Ultrasensitive Gold Nanoparticle-based Lateral Flow Test for the Detection of Active Botulinum Neurotoxin Type A
title_short An Ultrasensitive Gold Nanoparticle-based Lateral Flow Test for the Detection of Active Botulinum Neurotoxin Type A
title_full An Ultrasensitive Gold Nanoparticle-based Lateral Flow Test for the Detection of Active Botulinum Neurotoxin Type A
title_fullStr An Ultrasensitive Gold Nanoparticle-based Lateral Flow Test for the Detection of Active Botulinum Neurotoxin Type A
title_full_unstemmed An Ultrasensitive Gold Nanoparticle-based Lateral Flow Test for the Detection of Active Botulinum Neurotoxin Type A
title_sort ultrasensitive gold nanoparticle-based lateral flow test for the detection of active botulinum neurotoxin type a
publisher SpringerOpen
series Nanoscale Research Letters
issn 1931-7573
1556-276X
publishDate 2017-03-01
description Abstract Botulism is a severe and potentially lethal paralytic disease caused by several botulinum neurotoxin-producing Clostridia spp. In China, the majority of the cases caused by botulism were from less-developed rural areas. Here, we designed specific substrate peptides and reconfigured gold nanoparticle-based lateral flow test strip (LFTS) to develop an endopeptidase-based lateral flow assay for the diagnosis of botulism. We performed this lateral flow assay on botulinum neurotoxin-spiked human serum samples. The as-prepared LFTS had excellent performance in the detection of botulinum neurotoxin using only 1 μL of simulated serum, and its sensitivity and specificity were comparable to that of mouse lethality assay. Moreover, the assay takes only half a day and does not require highly trained laboratory staff, specialized facility, or equipment. Finally, our LFTS can be potentially extended to other serotypes of BoNTs by designing specific substrate peptides against the different types of BoNTs. Overall, we demonstrate a strategy by which LFTS and endopeptidase activity assays can be integrated to achieve facile and economic diagnosis of botulism in resource-limited settings.
topic Botulism
Botulinum toxin
Lateral flow assay
Endopeptidase activity assay
Mouse lethality assay
url http://link.springer.com/article/10.1186/s11671-017-1944-9
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