Investigation of low amyloid level toxicity effects on the function of hippocampal neurons

Alzheimer disease (AD) is responsible for the majority of elderly dementia cases. There are multiple mouse models of AD, however, none of them completely recapitulate human pathology. Moreover, it is even more difficult to imitate the onset of the disease in experimental models. In the current study...

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Main Authors:	Popugaeva Elena, Pchitskaya Ekaterina, Zhang Hua, Vlasova Olga, Bezprozvanny Ilya
Format:	Article
Language:	English
Published:	De Gruyter 2015-10-01
Series:	Open Life Sciences
Subjects:	Alzheimer disease amyloid beta calcium imaging
Online Access:	http://www.degruyter.com/view/j/biol.2015.10.issue-1/biol-2015-0045/biol-2015-0045.xml?format=INT

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spelling	doaj-3b2a914e835546f5bfe9d5dd7c0268fc2020-11-24T22:09:52ZengDe GruyterOpen Life Sciences2391-54122015-10-0110110.1515/biol-2015-0045biol-2015-0045Investigation of low amyloid level toxicity effects on the function of hippocampal neuronsPopugaeva Elena0Pchitskaya Ekaterina1Zhang Hua2Vlasova Olga3Bezprozvanny Ilya4Laboratory of Molecular Neurodegeneration, Department of Medical Physics, Peter the Great St. Petersburg Polytechnic University, Polytehnicheskaya 29, Saint Petersburg, 195251, Russian FederationLaboratory of Molecular Neurodegeneration, Department of Medical Physics, Peter the Great St. Petersburg Polytechnic University, Polytehnicheskaya 29, Saint Petersburg, 195251, Russian FederationDepartment of Physiology, UT Southwestern Medical Center at Dallas, Dallas, 75390-9040, Texas, USALaboratory of Molecular Neurodegeneration, Department of Medical Physics, Peter the Great St. Petersburg Polytechnic University, Polytehnicheskaya 29, Saint Petersburg, 195251, Russian FederationDepartment of Physiology, UT Southwestern Medical Center at Dallas, Dallas, 75390-9040, Texas, USAAlzheimer disease (AD) is responsible for the majority of elderly dementia cases. There are multiple mouse models of AD, however, none of them completely recapitulate human pathology. Moreover, it is even more difficult to imitate the onset of the disease in experimental models. In the current study we analyzed the influence of low amyloid level toxicity on the function of hippocampal neurons. Our assay is based on use of synthetic oligomeric amyloid beta peptides. These peptides were added to primary hippocampal cultures at a physiological nanomolar range to mimic early AD. Calcium imaging was used to evaluate the functionality of the assay. A low amyloid level toxicity assay could be useful for fundamental research as well as for testing newly developed AD drugs.http://www.degruyter.com/view/j/biol.2015.10.issue-1/biol-2015-0045/biol-2015-0045.xml?format=INTAlzheimer disease amyloid beta calcium imaging
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Popugaeva Elena Pchitskaya Ekaterina Zhang Hua Vlasova Olga Bezprozvanny Ilya
spellingShingle	Popugaeva Elena Pchitskaya Ekaterina Zhang Hua Vlasova Olga Bezprozvanny Ilya Investigation of low amyloid level toxicity effects on the function of hippocampal neurons Open Life Sciences Alzheimer disease amyloid beta calcium imaging
author_facet	Popugaeva Elena Pchitskaya Ekaterina Zhang Hua Vlasova Olga Bezprozvanny Ilya
author_sort	Popugaeva Elena
title	Investigation of low amyloid level toxicity effects on the function of hippocampal neurons
title_short	Investigation of low amyloid level toxicity effects on the function of hippocampal neurons
title_full	Investigation of low amyloid level toxicity effects on the function of hippocampal neurons
title_fullStr	Investigation of low amyloid level toxicity effects on the function of hippocampal neurons
title_full_unstemmed	Investigation of low amyloid level toxicity effects on the function of hippocampal neurons
title_sort	investigation of low amyloid level toxicity effects on the function of hippocampal neurons
publisher	De Gruyter
series	Open Life Sciences
issn	2391-5412
publishDate	2015-10-01
description	Alzheimer disease (AD) is responsible for the majority of elderly dementia cases. There are multiple mouse models of AD, however, none of them completely recapitulate human pathology. Moreover, it is even more difficult to imitate the onset of the disease in experimental models. In the current study we analyzed the influence of low amyloid level toxicity on the function of hippocampal neurons. Our assay is based on use of synthetic oligomeric amyloid beta peptides. These peptides were added to primary hippocampal cultures at a physiological nanomolar range to mimic early AD. Calcium imaging was used to evaluate the functionality of the assay. A low amyloid level toxicity assay could be useful for fundamental research as well as for testing newly developed AD drugs.
topic	Alzheimer disease amyloid beta calcium imaging
url	http://www.degruyter.com/view/j/biol.2015.10.issue-1/biol-2015-0045/biol-2015-0045.xml?format=INT
work_keys_str_mv	AT popugaevaelena investigationoflowamyloidleveltoxicityeffectsonthefunctionofhippocampalneurons AT pchitskayaekaterina investigationoflowamyloidleveltoxicityeffectsonthefunctionofhippocampalneurons AT zhanghua investigationoflowamyloidleveltoxicityeffectsonthefunctionofhippocampalneurons AT vlasovaolga investigationoflowamyloidleveltoxicityeffectsonthefunctionofhippocampalneurons AT bezprozvannyilya investigationoflowamyloidleveltoxicityeffectsonthefunctionofhippocampalneurons
_version_	1725810384096460800

Investigation of low amyloid level toxicity effects on the function of hippocampal neurons

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