Investigation of low amyloid level toxicity effects on the function of hippocampal neurons
Alzheimer disease (AD) is responsible for the majority of elderly dementia cases. There are multiple mouse models of AD, however, none of them completely recapitulate human pathology. Moreover, it is even more difficult to imitate the onset of the disease in experimental models. In the current study...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2015-10-01
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| Series: | Open Life Sciences |
| Subjects: | |
| Online Access: | http://www.degruyter.com/view/j/biol.2015.10.issue-1/biol-2015-0045/biol-2015-0045.xml?format=INT |
| Summary: | Alzheimer disease (AD) is responsible for the
majority of elderly dementia cases. There are multiple
mouse models of AD, however, none of them completely
recapitulate human pathology. Moreover, it is even more
difficult to imitate the onset of the disease in experimental
models. In the current study we analyzed the influence of
low amyloid level toxicity on the function of hippocampal
neurons. Our assay is based on use of synthetic oligomeric
amyloid beta peptides. These peptides were added to
primary hippocampal cultures at a physiological nanomolar
range to mimic early AD. Calcium imaging was used to
evaluate the functionality of the assay. A low amyloid level
toxicity assay could be useful for fundamental research as
well as for testing newly developed AD drugs. |
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| ISSN: | 2391-5412 |