Aquatic Training after Joint Immobilization in Rats Promotes Adaptations in Myotendinous Junctions

• Main Authors: Lara Caetano Rocha, Gabriela Klein Barbosa, Jurandyr Pimentel Neto, Carolina dos Santos Jacob, Andreas B. Knudsen, Ii-Sei Watanabe, Adriano Polican Ciena
• Format: Article
• Language: English
• Published: MDPI AG 2021-06-01
• Series: International Journal of Molecular Sciences
• Subjects: nuclear domain; sarcomere; telocyte; muscle-tendon perimeter; aquatic training; joint immobilization
• Online Access: https://www.mdpi.com/1422-0067/22/13/6983
• ISSN: 1661-6596; 1422-0067

The myotendinous junction (MTJ) is the muscle-tendon interface and constitutes an integrated mechanical unit to force transmission. Joint immobilization promotes muscle atrophy via disuse, while physical exercise can be used as an adaptative stimulus. In this study, we aimed to investigate the components of the MTJ and their adaptations and the associated elements triggered with aquatic training after joint immobilization. Forty-four male Wistar rats were divided into sedentary (SD), aquatic training (AT), immobilization (IM), and immobilization/aquatic training (IMAT) groups. The samples were processed to measure fiber area, nuclear fractal dimension, MTJ nuclear density, identification of telocytes, sarcomeres, and MTJ perimeter length. In the AT group, the maintenance of ultrastructure and elements in the MTJ region were observed; the IM group presented muscle atrophy effects with reduced MTJ perimeter; the IMAT group demonstrated that aquatic training after joint immobilization promotes benefits in the muscle fiber area and fractal dimension, in the MTJ region shows longer sarcomeres and MTJ perimeter. We identified the presence of telocytes in the MTJ region in all experimental groups. We concluded that aquatic training is an effective rehabilitation method after joint immobilization due to reduced muscle atrophy and regeneration effects on MTJ in rats.