NeuroCore formation during differentiation of neurospheres of mouse embryonic neural stem cells

Neural stem cells (NSCs) in the embryonic neocortex have the potential to generate a well-organized laminar architecture of the cerebral cortex through precise regulation of the proliferation, differentiation, and migration of neural cells. NSCs can be isolated in vitro and expanded as cell clusters...

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Main Authors: Ju-Hyun Lee, Mohammed R. Shaker, Eunsoo Lee, Boram Lee, Woong Sun
Format: Article
Language:English
Published: Elsevier 2020-03-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506119303216
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spelling doaj-3ab8eccc0c8d4f6d8327f6cd9c0dc7d32020-11-25T01:23:41ZengElsevierStem Cell Research1873-50612020-03-0143NeuroCore formation during differentiation of neurospheres of mouse embryonic neural stem cellsJu-Hyun Lee0Mohammed R. Shaker1Eunsoo Lee2Boram Lee3Woong Sun4Department of Anatomy and Division of Brain Korea 21 Plus Program for Biomedical Science, Korea University College of Medicine, 73, Inchon-ro, Seongbuk-gu, Seoul 02841, Republic of KoreaDepartment of Anatomy and Division of Brain Korea 21 Plus Program for Biomedical Science, Korea University College of Medicine, 73, Inchon-ro, Seongbuk-gu, Seoul 02841, Republic of KoreaDepartment of Anatomy and Division of Brain Korea 21 Plus Program for Biomedical Science, Korea University College of Medicine, 73, Inchon-ro, Seongbuk-gu, Seoul 02841, Republic of KoreaDepartment of Anatomy and Division of Brain Korea 21 Plus Program for Biomedical Science, Korea University College of Medicine, 73, Inchon-ro, Seongbuk-gu, Seoul 02841, Republic of KoreaCorresponding author.; Department of Anatomy and Division of Brain Korea 21 Plus Program for Biomedical Science, Korea University College of Medicine, 73, Inchon-ro, Seongbuk-gu, Seoul 02841, Republic of KoreaNeural stem cells (NSCs) in the embryonic neocortex have the potential to generate a well-organized laminar architecture of the cerebral cortex through precise regulation of the proliferation, differentiation, and migration of neural cells. NSCs can be isolated in vitro and expanded as cell clusters, called neurospheres, which are primarily related to the proliferation ability of NSCs. Conversely, the tissue-organizing properties of NSCs via regulated differentiation and migration of the cells are not well understood. In this study, we established a three-dimensional (3D) differentiation model of neurospheres, which produce unique neuronal clusters, termed NeuroCore (NC). NC formation was initiated by the aggregation of young neurons. Upon maturation of the neurons and the establishment of radial glia-like structures, the initial organization of the NCs transformed into a glomeruli-like arrangement of cortical neurons. These neurons expressed multiple markers of upper and deep cortical neurons. Taken together, we propose that NSCs in vitro maintain some aspects of their original in vivo tissue-organizing properties, providing an alternative opportunity to explore the fundamental components of brain histogenesis in vitro. Keywords: Neural stem cell, Neurosphere, Differentiation, Neocortexhttp://www.sciencedirect.com/science/article/pii/S1873506119303216
collection DOAJ
language English
format Article
sources DOAJ
author Ju-Hyun Lee
Mohammed R. Shaker
Eunsoo Lee
Boram Lee
Woong Sun
spellingShingle Ju-Hyun Lee
Mohammed R. Shaker
Eunsoo Lee
Boram Lee
Woong Sun
NeuroCore formation during differentiation of neurospheres of mouse embryonic neural stem cells
Stem Cell Research
author_facet Ju-Hyun Lee
Mohammed R. Shaker
Eunsoo Lee
Boram Lee
Woong Sun
author_sort Ju-Hyun Lee
title NeuroCore formation during differentiation of neurospheres of mouse embryonic neural stem cells
title_short NeuroCore formation during differentiation of neurospheres of mouse embryonic neural stem cells
title_full NeuroCore formation during differentiation of neurospheres of mouse embryonic neural stem cells
title_fullStr NeuroCore formation during differentiation of neurospheres of mouse embryonic neural stem cells
title_full_unstemmed NeuroCore formation during differentiation of neurospheres of mouse embryonic neural stem cells
title_sort neurocore formation during differentiation of neurospheres of mouse embryonic neural stem cells
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2020-03-01
description Neural stem cells (NSCs) in the embryonic neocortex have the potential to generate a well-organized laminar architecture of the cerebral cortex through precise regulation of the proliferation, differentiation, and migration of neural cells. NSCs can be isolated in vitro and expanded as cell clusters, called neurospheres, which are primarily related to the proliferation ability of NSCs. Conversely, the tissue-organizing properties of NSCs via regulated differentiation and migration of the cells are not well understood. In this study, we established a three-dimensional (3D) differentiation model of neurospheres, which produce unique neuronal clusters, termed NeuroCore (NC). NC formation was initiated by the aggregation of young neurons. Upon maturation of the neurons and the establishment of radial glia-like structures, the initial organization of the NCs transformed into a glomeruli-like arrangement of cortical neurons. These neurons expressed multiple markers of upper and deep cortical neurons. Taken together, we propose that NSCs in vitro maintain some aspects of their original in vivo tissue-organizing properties, providing an alternative opportunity to explore the fundamental components of brain histogenesis in vitro. Keywords: Neural stem cell, Neurosphere, Differentiation, Neocortex
url http://www.sciencedirect.com/science/article/pii/S1873506119303216
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