Synthetic control of plasmid replication enables target- and self-curing of vectors and expedites genome engineering of Pseudomonas putida

Genome engineering of non-conventional microorganisms calls for the development of dedicated synthetic biology tools. Pseudomonas putida is a Gram-negative, non-pathogenic soil bacterium widely used for metabolic engineering owing to its versatile metabolism and high levels of tolerance to different...

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Bibliographic Details
Main Authors: Daniel C. Volke, Laura Friis, Nicolas T. Wirth, Justine Turlin, Pablo I. Nikel
Format: Article
Language:English
Published: Elsevier 2020-06-01
Series:Metabolic Engineering Communications
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2214030120300018