Validación de una metodología multirresiduo para la determinación de residuos de plaguicidas en fresa (Fragraria spp. ) por cromatografía de gases

• Main Authors: Mauricio Montaño Garcés, Jairo Arturo Guerrero Dallos
• Format: Article
• Language: English
• Published: Universidad Nacional de Colombia 2011-03-01
• Series: Revista Colombiana de Química
• Subjects: plaguiciclas; fresa; método multirresiduo; cromatografía de gases; cromatografía de permeación en geles
• Online Access: http://www.revistas.unal.edu.co/index.php/rcolquim/article/view/22282

En este estudio se describe la validación de una metodología analítica multirresiduo para la determinación simultánea<br />de 19 compuestos organoclorados, organofosforados y organonitrogenados en fresa. Los plaguicidas se extrajeron<br />con acetato de etilo, los extractos se limpiaron por cromatografía de permeación por geles (GPC), el análisis se llevó a cabo por cromatografía de gases de alta resolución (CGAR) con inyección en modo splitless pulsado y detección simultánea por microcaptura electrónica (<!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:TrackMoves /> <w:TrackFormatting /> <w:HyphenationZone>21</w:HyphenationZone> <w:PunctuationKerning /> <w:ValidateAgainstSchemas /> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:DoNotPromoteQF /> <w:LidThemeOther>ES</w:LidThemeOther> <w:LidThemeAsian>X-NONE</w:LidThemeAsian> <w:LidThemeComplexScript>X-NONE</w:LidThemeComplexScript> <w:Compatibility> <w:BreakWrappedTables /> <w:SnapToGridInCell /> <w:WrapTextWithPunct /> <w:UseAsianBreakRules /> <w:DontGrowAutofit /> 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Name="Bibliography" /> <w:LsdException Locked="false" Priority="39" QFormat="true" Name="TOC Heading" /> </w:LatentStyles> </xml><![endif]--><!--[if gte mso 10]> <style> /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;} </style> <![endif]--><span style="font-family: ";Times New Roman";,";serif";;">μ</span>-ECD)y nitrógeno-fósforo (NPD) acopiadosen paralelo. La metodología es específica, selectiva, precisa y robusta. Las curvas de calibración en extracto de matriz fortificado son lineales en un rango de concentración de 0.04-5.00 mg/kg, con cantidad mínima detectable (CMD) entre 0.007-0.50 mg/kg y cantidad mínima cuantificable (CMC) entre 0.01-1.00 mg/kg. Los porcentajes de recuperación estuvieron entre el 80-110 %, en el rango de calibración para la mayoría de plaguicidas  evaluados. Con respecto a la distribución del analito en la muestra, se demostró su homogeneidad. Se aplicó la  metodología a muestras reales, encontrándose principalmente residuos de Captan por debajo de los límites máximos de residuos (LMR). <br />