Cross-species Transcriptomic Comparison of and Mammalian Neural Cells
The mammalian brain is characterized by distinct classes of cells that differ in morphology, structure, signaling, and function. Dysregulation of gene expression in these cell populations leads to various neurological disorders. Neural cells often need to be acutely purified from animal brains for r...
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doaj-3a7aa99cb8ee4f98a19e2d841389e9882020-11-25T03:41:16ZengSAGE PublishingBioinformatics and Biology Insights1177-93222015-01-01910.4137/BBI.S33124Cross-species Transcriptomic Comparison of and Mammalian Neural CellsPeter R. LoVerso0Christopher M. Wachter1Feng Cui2Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester, NY, USA.Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester, NY, USA.Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester, NY, USA.The mammalian brain is characterized by distinct classes of cells that differ in morphology, structure, signaling, and function. Dysregulation of gene expression in these cell populations leads to various neurological disorders. Neural cells often need to be acutely purified from animal brains for research, which requires complicated procedure and specific expertise. Primary culture of these cells in vitro is a viable alternative, but the differences in gene expression of cells grown in vitro and in vivo remain unclear. Here, we cultured three major neural cell classes of rat brain (ie, neurons, astrocytes, and oligodendrocyte precursor cells [OPCs]) obtained from commercial sources. We measured transcript abundance of these cell types by RNA sequencing (RNA-seq) and compared with their counterparts acutely purified from mouse brains. Cross-species RNA-seq data analysis revealed hundreds of genes that are differentially expressed between the cultured and acutely purified cells. Astrocytes have more such genes compared to neurons and OPCs, indicating that signaling pathways are greatly perturbed in cultured astrocytes. This dataset provides a powerful resource to demonstrate the similarities and differences of biological processes in mammalian neural cells grown in vitro and in vivo at the molecular level.https://doi.org/10.4137/BBI.S33124 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Peter R. LoVerso Christopher M. Wachter Feng Cui |
spellingShingle |
Peter R. LoVerso Christopher M. Wachter Feng Cui Cross-species Transcriptomic Comparison of and Mammalian Neural Cells Bioinformatics and Biology Insights |
author_facet |
Peter R. LoVerso Christopher M. Wachter Feng Cui |
author_sort |
Peter R. LoVerso |
title |
Cross-species Transcriptomic Comparison of and Mammalian Neural Cells |
title_short |
Cross-species Transcriptomic Comparison of and Mammalian Neural Cells |
title_full |
Cross-species Transcriptomic Comparison of and Mammalian Neural Cells |
title_fullStr |
Cross-species Transcriptomic Comparison of and Mammalian Neural Cells |
title_full_unstemmed |
Cross-species Transcriptomic Comparison of and Mammalian Neural Cells |
title_sort |
cross-species transcriptomic comparison of and mammalian neural cells |
publisher |
SAGE Publishing |
series |
Bioinformatics and Biology Insights |
issn |
1177-9322 |
publishDate |
2015-01-01 |
description |
The mammalian brain is characterized by distinct classes of cells that differ in morphology, structure, signaling, and function. Dysregulation of gene expression in these cell populations leads to various neurological disorders. Neural cells often need to be acutely purified from animal brains for research, which requires complicated procedure and specific expertise. Primary culture of these cells in vitro is a viable alternative, but the differences in gene expression of cells grown in vitro and in vivo remain unclear. Here, we cultured three major neural cell classes of rat brain (ie, neurons, astrocytes, and oligodendrocyte precursor cells [OPCs]) obtained from commercial sources. We measured transcript abundance of these cell types by RNA sequencing (RNA-seq) and compared with their counterparts acutely purified from mouse brains. Cross-species RNA-seq data analysis revealed hundreds of genes that are differentially expressed between the cultured and acutely purified cells. Astrocytes have more such genes compared to neurons and OPCs, indicating that signaling pathways are greatly perturbed in cultured astrocytes. This dataset provides a powerful resource to demonstrate the similarities and differences of biological processes in mammalian neural cells grown in vitro and in vivo at the molecular level. |
url |
https://doi.org/10.4137/BBI.S33124 |
work_keys_str_mv |
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