Specific association of lectin LecB with the surface of Pseudomonas aeruginosa: role of outer membrane protein OprF.

Specific association of lectin LecB with the surface of Pseudomonas aeruginosa: role of outer membrane protein OprF.

The fucose binding lectin LecB affects biofilm formation and is involved in pathogenicity of Pseudomonas aeruginosa. LecB resides in the outer membrane and can be released specifically by treatment of an outer membrane fraction with fucose suggesting that it binds to specific ligands. Here, we repor...

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Main Authors: Horst Funken, Kai-Malte Bartels, Susanne Wilhelm, Melanie Brocker, Michael Bott, Manjeet Bains, Robert E W Hancock, Frank Rosenau, Karl-Erich Jaeger
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3466170?pdf=render
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spelling doaj-3a674b52b31241e9858a1cd9110182802020-11-25T01:53:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01710e4685710.1371/journal.pone.0046857Specific association of lectin LecB with the surface of Pseudomonas aeruginosa: role of outer membrane protein OprF.Horst FunkenKai-Malte BartelsSusanne WilhelmMelanie BrockerMichael BottManjeet BainsRobert E W HancockFrank RosenauKarl-Erich JaegerThe fucose binding lectin LecB affects biofilm formation and is involved in pathogenicity of Pseudomonas aeruginosa. LecB resides in the outer membrane and can be released specifically by treatment of an outer membrane fraction with fucose suggesting that it binds to specific ligands. Here, we report that LecB binds to the outer membrane protein OprF. In an OprF-deficient P. aeruginosa mutant, LecB is no longer detectable in the membrane but instead in the culture supernatant indicating a specific interaction between LecB and OprF.http://europepmc.org/articles/PMC3466170?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Horst Funken
Kai-Malte Bartels
Susanne Wilhelm
Melanie Brocker
Michael Bott
Manjeet Bains
Robert E W Hancock
Frank Rosenau
Karl-Erich Jaeger
spellingShingle Horst Funken
Kai-Malte Bartels
Susanne Wilhelm
Melanie Brocker
Michael Bott
Manjeet Bains
Robert E W Hancock
Frank Rosenau
Karl-Erich Jaeger
Specific association of lectin LecB with the surface of Pseudomonas aeruginosa: role of outer membrane protein OprF.
PLoS ONE
author_facet Horst Funken
Kai-Malte Bartels
Susanne Wilhelm
Melanie Brocker
Michael Bott
Manjeet Bains
Robert E W Hancock
Frank Rosenau
Karl-Erich Jaeger
author_sort Horst Funken
title Specific association of lectin LecB with the surface of Pseudomonas aeruginosa: role of outer membrane protein OprF.
title_short Specific association of lectin LecB with the surface of Pseudomonas aeruginosa: role of outer membrane protein OprF.
title_full Specific association of lectin LecB with the surface of Pseudomonas aeruginosa: role of outer membrane protein OprF.
title_fullStr Specific association of lectin LecB with the surface of Pseudomonas aeruginosa: role of outer membrane protein OprF.
title_full_unstemmed Specific association of lectin LecB with the surface of Pseudomonas aeruginosa: role of outer membrane protein OprF.
title_sort specific association of lectin lecb with the surface of pseudomonas aeruginosa: role of outer membrane protein oprf.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2012-01-01
description The fucose binding lectin LecB affects biofilm formation and is involved in pathogenicity of Pseudomonas aeruginosa. LecB resides in the outer membrane and can be released specifically by treatment of an outer membrane fraction with fucose suggesting that it binds to specific ligands. Here, we report that LecB binds to the outer membrane protein OprF. In an OprF-deficient P. aeruginosa mutant, LecB is no longer detectable in the membrane but instead in the culture supernatant indicating a specific interaction between LecB and OprF.
url http://europepmc.org/articles/PMC3466170?pdf=render
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