Lost Polarization of Aquaporin4 and Dystroglycan in the Core Lesion after Traumatic Brain Injury Suggests Functional Divergence in Evolution

Objective. To understand how aquaporin4 (AQP4) and dystroglycan (DG) polarized distribution change and their roles in brain edema formation after traumatic brain injury (TBI). Methods. Brain water content, Evans blue detection, real-time PCR, western blot, and immunofluorescence were used. Results....

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Main Authors: Hui Liu, Gou ping Qiu, Fei Zhuo, Wei hua Yu, Shan quan Sun, Fen hong Li, Mei Yang
Format: Article
Language:English
Published: Hindawi Limited 2015-01-01
Series:BioMed Research International
Online Access:http://dx.doi.org/10.1155/2015/471631
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spelling doaj-3a66b750294e4607b4627ed59b598d6b2020-11-24T22:51:08ZengHindawi LimitedBioMed Research International2314-61332314-61412015-01-01201510.1155/2015/471631471631Lost Polarization of Aquaporin4 and Dystroglycan in the Core Lesion after Traumatic Brain Injury Suggests Functional Divergence in EvolutionHui Liu0Gou ping Qiu1Fei Zhuo2Wei hua Yu3Shan quan Sun4Fen hong Li5Mei Yang6Institute of Neuroscience, Chongqing Medical University, Chongqing 400016, ChinaInstitute of Neuroscience, Chongqing Medical University, Chongqing 400016, ChinaInstitute of Neuroscience, Chongqing Medical University, Chongqing 400016, ChinaInstitute of Neuroscience, Chongqing Medical University, Chongqing 400016, ChinaInstitute of Neuroscience, Chongqing Medical University, Chongqing 400016, ChinaDepartment of Mathematics and Computation Science, Shangluo University, Shangluo, Shanxi 726000, ChinaInstitute of Neuroscience, Chongqing Medical University, Chongqing 400016, ChinaObjective. To understand how aquaporin4 (AQP4) and dystroglycan (DG) polarized distribution change and their roles in brain edema formation after traumatic brain injury (TBI). Methods. Brain water content, Evans blue detection, real-time PCR, western blot, and immunofluorescence were used. Results. At an early stage of TBI, AQP4 and DG maintained vessel-like pattern in perivascular endfeet; M1, M23, and M1/M23 were increased in the core lesion. At a later stage of TBI, DG expression was lost in perivascular area, accompanied with similar but delayed change of AQP4 expression; expression of M1, M23, and DG and the ratio of M1/M2 were increased. Conclusion. At an early stage, AQP4 and DG maintained the polarized distribution. Upregulated M1 and M23 could retard the cytotoxic edema formation. At a later stage AQP4 and DG polarized expression were lost from perivascular endfeet and induced the worst cytotoxic brain edema. The alteration of DG expression could regulate that of AQP4 expression after TBI.http://dx.doi.org/10.1155/2015/471631
collection DOAJ
language English
format Article
sources DOAJ
author Hui Liu
Gou ping Qiu
Fei Zhuo
Wei hua Yu
Shan quan Sun
Fen hong Li
Mei Yang
spellingShingle Hui Liu
Gou ping Qiu
Fei Zhuo
Wei hua Yu
Shan quan Sun
Fen hong Li
Mei Yang
Lost Polarization of Aquaporin4 and Dystroglycan in the Core Lesion after Traumatic Brain Injury Suggests Functional Divergence in Evolution
BioMed Research International
author_facet Hui Liu
Gou ping Qiu
Fei Zhuo
Wei hua Yu
Shan quan Sun
Fen hong Li
Mei Yang
author_sort Hui Liu
title Lost Polarization of Aquaporin4 and Dystroglycan in the Core Lesion after Traumatic Brain Injury Suggests Functional Divergence in Evolution
title_short Lost Polarization of Aquaporin4 and Dystroglycan in the Core Lesion after Traumatic Brain Injury Suggests Functional Divergence in Evolution
title_full Lost Polarization of Aquaporin4 and Dystroglycan in the Core Lesion after Traumatic Brain Injury Suggests Functional Divergence in Evolution
title_fullStr Lost Polarization of Aquaporin4 and Dystroglycan in the Core Lesion after Traumatic Brain Injury Suggests Functional Divergence in Evolution
title_full_unstemmed Lost Polarization of Aquaporin4 and Dystroglycan in the Core Lesion after Traumatic Brain Injury Suggests Functional Divergence in Evolution
title_sort lost polarization of aquaporin4 and dystroglycan in the core lesion after traumatic brain injury suggests functional divergence in evolution
publisher Hindawi Limited
series BioMed Research International
issn 2314-6133
2314-6141
publishDate 2015-01-01
description Objective. To understand how aquaporin4 (AQP4) and dystroglycan (DG) polarized distribution change and their roles in brain edema formation after traumatic brain injury (TBI). Methods. Brain water content, Evans blue detection, real-time PCR, western blot, and immunofluorescence were used. Results. At an early stage of TBI, AQP4 and DG maintained vessel-like pattern in perivascular endfeet; M1, M23, and M1/M23 were increased in the core lesion. At a later stage of TBI, DG expression was lost in perivascular area, accompanied with similar but delayed change of AQP4 expression; expression of M1, M23, and DG and the ratio of M1/M2 were increased. Conclusion. At an early stage, AQP4 and DG maintained the polarized distribution. Upregulated M1 and M23 could retard the cytotoxic edema formation. At a later stage AQP4 and DG polarized expression were lost from perivascular endfeet and induced the worst cytotoxic brain edema. The alteration of DG expression could regulate that of AQP4 expression after TBI.
url http://dx.doi.org/10.1155/2015/471631
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