Renoprotective effects of enzyme-hydrolyzed polysaccharides from Auricularia polytricha on adenine-induced chronic kidney diseases in mice

• Main Authors: Xinling Song, Hui Pang, Weijun Cui, Jianjun Zhang, Jian Li, Le Jia
• Format: Article
• Language: English
• Published: Elsevier 2021-03-01
• Series: Biomedicine & Pharmacotherapy
• Subjects: Auricularia polytricha polysaccharide; Antioxidant; Anti-inflammation; Anti-apoptosis; Chronic kidney disease; Renoprotection
• Online Access: http://www.sciencedirect.com/science/article/pii/S0753332220311963

The present work was aimed to investigate the protective effects of enzymatic-hydrolyzed Auricularia polytricha polysaccharides (EnAPS) on renal functions. The characterizations were analyzed by physicochemical methods, and the renoprotections were processed in adenine-induced chronic kidney diseases (CKD) models of mice. Animal experiments exhibited that EnAPS showed superior renal-protections contributing to its antioxidant effects of increasing the enzyme activities and decreasing the lipid contents, and anti-inflammatory effects of reducing proinflammatory cytokines than A. polytricha polysaccharides (APS). Besides, the anti-apoptosis effects of EnAPS was proved by down-regulating Bax and Caspase-3 expressions and up-regulating Bcl-2 expressions by molecular biotechnology, and the anti-fibrosis effects was confirmed by histopathological observations of staining. The characterizations indicated that lower molecular weights possibly contributed to the superior renoprotective effects. These results suggested that enzymatic hydrolysis had potential effects in enhancing the bioactivities, and the polysaccharides could be used in the development of functional foods supplement against CKD.