Impact of repeated nasal sampling on detection and quantification of SARS-CoV-2

Impact of repeated nasal sampling on detection and quantification of SARS-CoV-2

Abstract The impact of repeated sample collection on COVID-19 test performance is unknown. The FDA and CDC currently recommend the primary collection of diagnostic samples to minimize the perceived risk of false-negative findings. We therefore evaluated the association between repeated sample collec...

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Main Authors: Joshua M. Levy, Jennifer K. Frediani, Erika A. Tyburski, Anna Wood, Janet Figueroa, Russell R. Kempker, Paulina A. Rebolledo, Mark D. Gonzalez, Julie Sullivan, Miriam B. Vos, Jared O’Neal, Greg S. Martin, Wilbur A. Lam, Jesse J. Waggoner
Format: Article
Language:English
Published: Nature Publishing Group 2021-07-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-94547-0
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spelling doaj-39dfb0e9ecc74fe6a6c372c4079c88bd2021-07-25T11:24:37ZengNature Publishing GroupScientific Reports2045-23222021-07-011111510.1038/s41598-021-94547-0Impact of repeated nasal sampling on detection and quantification of SARS-CoV-2Joshua M. Levy0Jennifer K. Frediani1Erika A. Tyburski2Anna Wood3Janet Figueroa4Russell R. Kempker5Paulina A. Rebolledo6Mark D. Gonzalez7Julie Sullivan8Miriam B. Vos9Jared O’Neal10Greg S. Martin11Wilbur A. Lam12Jesse J. Waggoner13The Atlanta Center for Microsystems-Engineered Point-of-Care TechnologiesDepartment of Otolaryngology-Head and Neck Surgery, Emory University School of MedicineThe Atlanta Center for Microsystems-Engineered Point-of-Care TechnologiesDepartment of Pediatrics, Emory University School of MedicineDepartment of Pediatrics, Emory University School of MedicineThe Atlanta Center for Microsystems-Engineered Point-of-Care TechnologiesThe Atlanta Center for Microsystems-Engineered Point-of-Care TechnologiesThe Atlanta Center for Microsystems-Engineered Point-of-Care TechnologiesThe Atlanta Center for Microsystems-Engineered Point-of-Care TechnologiesThe Atlanta Center for Microsystems-Engineered Point-of-Care TechnologiesThe Atlanta Center for Microsystems-Engineered Point-of-Care TechnologiesThe Atlanta Center for Microsystems-Engineered Point-of-Care TechnologiesThe Atlanta Center for Microsystems-Engineered Point-of-Care TechnologiesThe Atlanta Center for Microsystems-Engineered Point-of-Care TechnologiesAbstract The impact of repeated sample collection on COVID-19 test performance is unknown. The FDA and CDC currently recommend the primary collection of diagnostic samples to minimize the perceived risk of false-negative findings. We therefore evaluated the association between repeated sample collection and test performance among 325 symptomatic patients undergoing COVID-19 testing in Atlanta, GA. High concordance was found between consecutively collected mid-turbinate samples with both molecular (n = 74, 100% concordance) and antigen-based (n = 147, 97% concordance, kappa = 0.95, CI = 0.88–1.00) diagnostic assays. Repeated sample collection does not decrease COVID-19 test performance, demonstrating that multiple samples can be collected for assay validation and clinical diagnosis.https://doi.org/10.1038/s41598-021-94547-0
collection DOAJ
language English
format Article
sources DOAJ
author Joshua M. Levy
Jennifer K. Frediani
Erika A. Tyburski
Anna Wood
Janet Figueroa
Russell R. Kempker
Paulina A. Rebolledo
Mark D. Gonzalez
Julie Sullivan
Miriam B. Vos
Jared O’Neal
Greg S. Martin
Wilbur A. Lam
Jesse J. Waggoner
spellingShingle Joshua M. Levy
Jennifer K. Frediani
Erika A. Tyburski
Anna Wood
Janet Figueroa
Russell R. Kempker
Paulina A. Rebolledo
Mark D. Gonzalez
Julie Sullivan
Miriam B. Vos
Jared O’Neal
Greg S. Martin
Wilbur A. Lam
Jesse J. Waggoner
Impact of repeated nasal sampling on detection and quantification of SARS-CoV-2
Scientific Reports
author_facet Joshua M. Levy
Jennifer K. Frediani
Erika A. Tyburski
Anna Wood
Janet Figueroa
Russell R. Kempker
Paulina A. Rebolledo
Mark D. Gonzalez
Julie Sullivan
Miriam B. Vos
Jared O’Neal
Greg S. Martin
Wilbur A. Lam
Jesse J. Waggoner
author_sort Joshua M. Levy
title Impact of repeated nasal sampling on detection and quantification of SARS-CoV-2
title_short Impact of repeated nasal sampling on detection and quantification of SARS-CoV-2
title_full Impact of repeated nasal sampling on detection and quantification of SARS-CoV-2
title_fullStr Impact of repeated nasal sampling on detection and quantification of SARS-CoV-2
title_full_unstemmed Impact of repeated nasal sampling on detection and quantification of SARS-CoV-2
title_sort impact of repeated nasal sampling on detection and quantification of sars-cov-2
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2021-07-01
description Abstract The impact of repeated sample collection on COVID-19 test performance is unknown. The FDA and CDC currently recommend the primary collection of diagnostic samples to minimize the perceived risk of false-negative findings. We therefore evaluated the association between repeated sample collection and test performance among 325 symptomatic patients undergoing COVID-19 testing in Atlanta, GA. High concordance was found between consecutively collected mid-turbinate samples with both molecular (n = 74, 100% concordance) and antigen-based (n = 147, 97% concordance, kappa = 0.95, CI = 0.88–1.00) diagnostic assays. Repeated sample collection does not decrease COVID-19 test performance, demonstrating that multiple samples can be collected for assay validation and clinical diagnosis.
url https://doi.org/10.1038/s41598-021-94547-0
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