Optimization of heavy chain and light chain signal peptides for high level expression of therapeutic antibodies in CHO cells.

Optimization of heavy chain and light chain signal peptides for high level expression of therapeutic antibodies in CHO cells.

Translocation of a nascent protein from the cytosol into the ER mediated by its signal peptide is a critical step in protein secretion. The aim of this work was to develop a platform technology to optimize the signal peptides for high level production of therapeutic antibodies in CHO cells. A databa...

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Bibliographic Details
Main Authors: Ryan Haryadi, Steven Ho, Yee Jiun Kok, Helen X Pu, Lu Zheng, Natasha A Pereira, Bin Li, Xuezhi Bi, Lin-Tang Goh, Yuansheng Yang, Zhiwei Song
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4338144?pdf=render

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