Identification of appropriate reference genes for human mesenchymal cells during expansion and differentiation.
BACKGROUND: Quantitative real time polymerase chain reaction (qPCR) is an extremely powerful technique for monitoring gene expression. The quantity of the messenger ribonucleic acids (mRNA) of interest should be normalized using a reference gene, in order to avoid unreliable results originated by th...
Main Authors: | Paola Romina Amable, Marcus Vinicius Telles Teixeira, Rosana Bizon Vieira Carias, José Mauro Granjeiro, Radovan Borojevic |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2013-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3759474?pdf=render |
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