Cloning, chromosome mapping and expression pattern of porcine <it>PLIN </it>and <it>M6PRBP1 </it>genes

• Main Authors: Yang Zaiqing, Peichao Zhang, Xiaodong Chen, Yi Zhu, Bin Feng, Li Gan, Jihong Yuan, Tao Xia
• Format: Article
• Language: deu
• Published: BMC 2008-03-01
• Series: Genetics Selection Evolution
• Subjects: pig; <it>PLIN</it>; <it>M6PRBP1</it>; cDNA cloning; chromosome mapping; tissue expression pattern
• Online Access: http://www.gsejournal.org/content/40/2/215

<p>Abstract</p> <p>The PAT proteins, named after the three PLIN/ADRP/TIP47 (PAT) proteins, PLIN for perilipin, ADRP for adipose differentiation-related protein and TIP47 for tail-interacting protein of 47 kDa, now officially named M6PRBP1 for mannose-6-phosphate receptor binding protein 1, is a set of intracellular lipid droplet binding proteins. They are localized in the outer membrane monolayer enveloping lipid droplets and are involved in the metabolism of intracellular lipid. This work describes the cloning and sequencing of porcine <it>PLIN </it>and <it>M6PRBP1 </it>cDNAs, the chromosome mapping of these two genes, as well as the expression pattern of porcine PAT genes. Sequence analysis shows that the porcine <it>PLIN </it>cDNA contains an open reading frame of 1551 bp encoding 516 amino acids and that the porcine <it>M6PRBP1 </it>cDNA contains a coding region of 1320 bp encoding 439 amino acids. Comparison of PLIN and M6PRBP1 amino-acid sequences among various species reveals that porcine and bovine proteins are the most conserved. Porcine <it>PLIN </it>and <it>M6PRBP1 </it>genes have been mapped to pig chromosomes 7 and 2, respectively, by radiation hybrid analysis using the IMpRH panel. Expression analyses in pig showed a high expression of <it>PLIN </it>mRNA in adipose tissue, <it>M6PRBP1 </it>mRNA in small intestine, kidney and spleen and <it>ADRP </it>mRNA in adipose tissue, lung and spleen.</p>