The crystal structure of the β subunit of luteinizing hormone and a model for the intact hormone

The β subunit of bovine luteinizing hormone (LH) was crystallized and its structure solved to 3.15 Å resolution by molecular replacement using human chorionic gonadotropin (hCG) β subunit as search model. The asymmetric unit contains two copies of the β subunit that are related by a non-crystallogr...

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Main Authors:	Steven B. Larson, Alexander McPherson
Format:	Article
Language:	English
Published:	Elsevier 2019-11-01
Series:	Current Research in Structural Biology
Subjects:	Gonadotropic hormone Pituitary hormone Reproduction Crystallography Glycosylation Proteolysis
Online Access:	http://www.sciencedirect.com/science/article/pii/S2665928X19300017

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spelling	doaj-3967ba76cdb042c7ac23b74e6b1ade3f2020-11-25T03:57:28ZengElsevierCurrent Research in Structural Biology2665-928X2019-11-01115The crystal structure of the β subunit of luteinizing hormone and a model for the intact hormoneSteven B. Larson0Alexander McPherson1Dept. Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA, USACorresponding author.; Dept. Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA, USAThe β subunit of bovine luteinizing hormone (LH) was crystallized and its structure solved to 3.15 Å resolution by molecular replacement using human chorionic gonadotropin (hCG) β subunit as search model. The asymmetric unit contains two copies of the β subunit that are related by a non-crystallographic symmetry (NCS) two-fold axis, both copies of which contain proteolytic cleavages after amino acid 100. It is noteworthy that the oligosaccharide moieties covalently attached at asparagine 13 were particularly pronounced in the electron density, allowing seven sugar residues to be defined. The α subunit of LH, which is common to all glycosylated gonadotropin hormones, was placed by superposition of hCG on the LH beta subunits, thereby yielding a model for the intact hormone.http://www.sciencedirect.com/science/article/pii/S2665928X19300017Gonadotropic hormonePituitary hormoneReproductionCrystallographyGlycosylationProteolysis
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Steven B. Larson Alexander McPherson
spellingShingle	Steven B. Larson Alexander McPherson The crystal structure of the β subunit of luteinizing hormone and a model for the intact hormone Current Research in Structural Biology Gonadotropic hormone Pituitary hormone Reproduction Crystallography Glycosylation Proteolysis
author_facet	Steven B. Larson Alexander McPherson
author_sort	Steven B. Larson
title	The crystal structure of the β subunit of luteinizing hormone and a model for the intact hormone
title_short	The crystal structure of the β subunit of luteinizing hormone and a model for the intact hormone
title_full	The crystal structure of the β subunit of luteinizing hormone and a model for the intact hormone
title_fullStr	The crystal structure of the β subunit of luteinizing hormone and a model for the intact hormone
title_full_unstemmed	The crystal structure of the β subunit of luteinizing hormone and a model for the intact hormone
title_sort	crystal structure of the β subunit of luteinizing hormone and a model for the intact hormone
publisher	Elsevier
series	Current Research in Structural Biology
issn	2665-928X
publishDate	2019-11-01
description	The β subunit of bovine luteinizing hormone (LH) was crystallized and its structure solved to 3.15 Å resolution by molecular replacement using human chorionic gonadotropin (hCG) β subunit as search model. The asymmetric unit contains two copies of the β subunit that are related by a non-crystallographic symmetry (NCS) two-fold axis, both copies of which contain proteolytic cleavages after amino acid 100. It is noteworthy that the oligosaccharide moieties covalently attached at asparagine 13 were particularly pronounced in the electron density, allowing seven sugar residues to be defined. The α subunit of LH, which is common to all glycosylated gonadotropin hormones, was placed by superposition of hCG on the LH beta subunits, thereby yielding a model for the intact hormone.
topic	Gonadotropic hormone Pituitary hormone Reproduction Crystallography Glycosylation Proteolysis
url	http://www.sciencedirect.com/science/article/pii/S2665928X19300017
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The crystal structure of the β subunit of luteinizing hormone and a model for the intact hormone

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