The Effect of Particle Size on the Deposition of Solid Lipid Nanoparticles in Different Skin Layers: A Histological Study

• Main Authors: Zahra Mardhiah Adib, Saeed Ghanbarzadeh, Maryam Kouhsoltani, Ahmad Yari Khosroshahi, Hamed Hamishehkar
• Format: Article
• Language: English
• Published: Tabriz University of Medical Sciences 2016-03-01
• Series: Advanced Pharmaceutical Bulletin
• Subjects: Solid lipid nanoparticle; SLN; Topical drug delivery; Skin penetration; Rhodamine B
• Online Access: http://journals.tbzmed.ac.ir/APB/Manuscript/APB-6-31.pdf

Purpose: In the present study the effect of particle size, as a substantial parameters in skin penetration, on the deposition depth and rate of SLNs in different layers of skin was explored. Methods: SLNs in different particle size ranges (80, 333 and 971 nm) made of Precirol as solid lipid were prepared using hot melt homogenization technique and pigmented by Rhodamine B to be able to be tracked in the skin under inspection of fluorescent microscopy. After 0.5 h, 3 h, 6 h and 24 h of SLNs administration on rat skin, animals were sacrificed and exercised skins were sliced by a freeze microtome. SLNs were monitored in the skin structure under fluorescence microscope. Results: The size of SLNs played a crucial role in the penetration to deep skin layers. The sub100 nm size range of SLNs showed the most promising skin penetration rate and depth mainly via hair follicles. Conclusion: The results of the present study indicated that the selection of an appropriate size of particles may be a valuable factor impacting the therapeutic outcomes of dermal drug administration.