In situ dissection of domain boundaries affect genome topology and gene transcription in Drosophila

In situ dissection of domain boundaries affect genome topology and gene transcription in Drosophila

In Drosophila, transcription is thought to be required for TAD formation, while the role of architectural proteins remains controversial. Here, the authors find that deletion of domain boundaries at the fly Notch locus results in TAD fusion and long-range topological defects, loss of architectural p...

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Main Authors: Rodrigo G. Arzate-Mejía, Angel Josué Cerecedo-Castillo, Georgina Guerrero, Mayra Furlan-Magaril, Félix Recillas-Targa
Format: Article
Language:English
Published: Nature Publishing Group 2020-02-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-14651-z
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spelling doaj-393e8b7027a242d192e00adcd68c20932021-05-11T08:56:23ZengNature Publishing GroupNature Communications2041-17232020-02-0111111610.1038/s41467-020-14651-zIn situ dissection of domain boundaries affect genome topology and gene transcription in DrosophilaRodrigo G. Arzate-Mejía0Angel Josué Cerecedo-Castillo1Georgina Guerrero2Mayra Furlan-Magaril3Félix Recillas-Targa4Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de MéxicoDepartamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de MéxicoDepartamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de MéxicoDepartamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de MéxicoDepartamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de MéxicoIn Drosophila, transcription is thought to be required for TAD formation, while the role of architectural proteins remains controversial. Here, the authors find that deletion of domain boundaries at the fly Notch locus results in TAD fusion and long-range topological defects, loss of architectural protein and RNA Pol II chromatin binding, and transcription defects.https://doi.org/10.1038/s41467-020-14651-z
collection DOAJ
language English
format Article
sources DOAJ
author Rodrigo G. Arzate-Mejía
Angel Josué Cerecedo-Castillo
Georgina Guerrero
Mayra Furlan-Magaril
Félix Recillas-Targa
spellingShingle Rodrigo G. Arzate-Mejía
Angel Josué Cerecedo-Castillo
Georgina Guerrero
Mayra Furlan-Magaril
Félix Recillas-Targa
In situ dissection of domain boundaries affect genome topology and gene transcription in Drosophila
Nature Communications
author_facet Rodrigo G. Arzate-Mejía
Angel Josué Cerecedo-Castillo
Georgina Guerrero
Mayra Furlan-Magaril
Félix Recillas-Targa
author_sort Rodrigo G. Arzate-Mejía
title In situ dissection of domain boundaries affect genome topology and gene transcription in Drosophila
title_short In situ dissection of domain boundaries affect genome topology and gene transcription in Drosophila
title_full In situ dissection of domain boundaries affect genome topology and gene transcription in Drosophila
title_fullStr In situ dissection of domain boundaries affect genome topology and gene transcription in Drosophila
title_full_unstemmed In situ dissection of domain boundaries affect genome topology and gene transcription in Drosophila
title_sort in situ dissection of domain boundaries affect genome topology and gene transcription in drosophila
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2020-02-01
description In Drosophila, transcription is thought to be required for TAD formation, while the role of architectural proteins remains controversial. Here, the authors find that deletion of domain boundaries at the fly Notch locus results in TAD fusion and long-range topological defects, loss of architectural protein and RNA Pol II chromatin binding, and transcription defects.
url https://doi.org/10.1038/s41467-020-14651-z
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AT angeljosuecerecedocastillo insitudissectionofdomainboundariesaffectgenometopologyandgenetranscriptionindrosophila
AT georginaguerrero insitudissectionofdomainboundariesaffectgenometopologyandgenetranscriptionindrosophila
AT mayrafurlanmagaril insitudissectionofdomainboundariesaffectgenometopologyandgenetranscriptionindrosophila
AT felixrecillastarga insitudissectionofdomainboundariesaffectgenometopologyandgenetranscriptionindrosophila
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