Detection of Rotavirus in Sewage and Creek Water: Efficiency of the Concentration Method

Detection of Rotavirus in Sewage and Creek Water: Efficiency of the Concentration Method

Simian rotavirus SA-11, experimentally seeded, was recovered from raw domestic sewage by a two-step concentration procedure, using filtration through a positively charged microporous filter (Zeta Plus 60 S) followed by ultracentrifugation, effecting an 8,000-fold concentration. By this method, a mea...

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Bibliographic Details
Main Authors: Mehnert DU, Stewien KE, Hársi CM, Queiroz APS, Candeias JMG, Candeias JAN
Format: Article
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 1997-01-01
Series:Memórias do Instituto Oswaldo Cruz.
Subjects:
rotavirus
sewage
virus concentration
direct immunoperoxidase
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000100020
Description
Summary:Simian rotavirus SA-11, experimentally seeded, was recovered from raw domestic sewage by a two-step concentration procedure, using filtration through a positively charged microporous filter (Zeta Plus 60 S) followed by ultracentrifugation, effecting an 8,000-fold concentration. By this method, a mean recovery of 81% ± 7.5 of the SA-11 virus, was achieved
ISSN:0074-0276
1678-8060

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