Evaluation of Cr(VI) Reduction Using Indigenous Bacterial Consortium Isolated from a Municipal Wastewater Sludge: Batch and Kinetic Studies

• Main Authors: Buyisile Kholisa, Mpumelelo Matsena, Evans M. N. Chirwa
• Format: Article
• Language: English
• Published: MDPI AG 2021-09-01
• Series: Catalysts
• Subjects: sludge cultures; culture characterization; Cr(VI) reduction kinetics; soil remediation; phylogenetic analysis; toxic metalloids
• Online Access: https://www.mdpi.com/2073-4344/11/9/1100

Hexavalent Chromium (Cr(VI)) has long been known to be highly mobile and toxic when compared with the other stable oxidation state, Cr(III). Cr(VI)-soluble environmental pollutants have been detected in soils and water bodies receiving industrial and agricultural waste. The reduction of Cr(VI) by microbial organisms is considered to be an environmentally compatible, less expensive and sustainable remediation alternative when compared to conventional treatment methods, such as chemical neutralization and chemical precipitation of Cr. This study aims to isolate and identify the composition of the microbial consortium culture isolated from waste activated sludge and digested sludge from a local wastewater treatment plant receiving high loads of Cr(VI) from an abandoned chrome foundry in Brits (North Waste Province, South Africa). Furthermore, the Cr(VI) reduction capability and efficiency by the isolated bacteria were investigated under a range of operational conditions, i.e., pH, temperature and Cr(VI) loading. The culture showed great efficiency in reduction capability, with 100% removal in less than 4 h at a nominal loading concentration of 50 mg Cr(VI)/L. The culture showed resilience by achieving total removal at concentrations as high as 400 mg Cr(VI)/L. The consortia exhibited considerable Cr(VI) removal efficiency in the pH range from 2 to 11, with 100% removal being achieved at a pH value of 7 at a 37 ± 1 °C incubation temperature. The time course reduction data fitted well on both first and second-order exponential rate equation yielding first-order rate constants in the range 0.615 to 0.011 h⁻¹ and second order rate constants 0.0532 to 5 × 10⁻⁵ L·mg⁻¹·h⁻¹ for Cr(VI) concentration of 50–400 mg/L. This study demonstrated the bacterial consortium from municipal wastewater sludge has a high tolerance and reduction ability over a wide range of experimental conditions. Thus, show promise that bacteria could be used for hexavalent chromium remediate in contaminated sites.