Cas9/gRNA-mediated genome editing of yeast mitochondria and Chlamydomonas chloroplasts
We present a new approach to edit both mitochondrial and chloroplast genomes. Organelles have been considered off-limits to CRISPR due to their impermeability to most RNA and DNA. This has prevented applications of Cas9/gRNA-mediated genome editing in organelles while the tool has been widely used f...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2020-01-01
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| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/8362.pdf |