Differential Antimicrobial Effect of Essential Oils and Their Main Components: Insights Based on the Cell Membrane and External Structure

• Main Authors: Sergio Andrade-Ochoa, Karla Fabiola Chacón-Vargas, Luvia Enid Sánchez-Torres, Blanca Estela Rivera-Chavira, Benjamín Nogueda-Torres, Guadalupe Virginia Nevárez-Moorillón
• Format: Article
• Language: English
• Published: MDPI AG 2021-05-01
• Series: Membranes
• Subjects: essential oils; terpenoids; phenylpropanoids; cell membranes; antimicrobial; mechanism of action
• Online Access: https://www.mdpi.com/2077-0375/11/6/405

The biological activity of essential oils and their major components is well documented. Essential oils such as oregano and cinnamon are known for their effect against bacteria, fungi, and even viruses. The mechanism of action is proposed to be related to membrane and external cell structures, including cell walls. This study aimed to evaluate the biological activity of seven essential oils and eight of their major components against Gram-negative and Gram-positive bacteria, filamentous fungi, and protozoans. The antimicrobial activity was evaluated by determination of the Minimal Inhibitory Concentration for <i>Bacillus cereus</i>, <i>Staphylococcus aureus</i>, <i>Listeria monocytogenes</i>, <i>Escherichia coli</i>, <i>Salmonella</i> Typhimurium, <i>Shigella sonnei</i>, <i>Aspergillus niger</i>, <i>Aspergillus ochraceus</i>, <i>Alternaria alternata,</i> and <i>Fusarium oxysporium</i>, the half-maximal inhibitory concentration (IC₅₀) for <i>Trypanosoma cruzi</i> and <i>Leishmania mexicana</i>, and the median lethal dose (LD₅₀) for <i>Giardia lamblia</i>. Results showed that oregano essential oil showed the best antibacterial activity (66–100 µg/mL), while cinnamon essential oil had the best fungicidal activity (66–116 µg/mL), and both showed excellent antiprotozoal activity (22–108 µg/mL). Regarding the major components, thymol and carvacrol were also good antimicrobials (23–200 µg/mL), and cinnamaldehyde was an antifungal compound (41–75 µg/mL). The major components were grouped according to their chemical structure as phenylpropanoids, terpenoids, and terpinenes. The statistical analysis of the grouped data demonstrated that protozoans were more susceptible to the essential oils, followed by fungi, Gram-positive bacteria, and Gram-negative bacteria. The analysis for the major components showed that the most resistant microbial group was fungi, which was followed by bacteria, and protozoans were also more susceptible. Principal Component Analysis for the essential oils demonstrated the relationship between the biological activity and the microbial group tested, with the first three components explaining 94.3% of the data variability. The chemical structure of the major components was also related to the biological activity presented against the microbial groups tested, where the three first principal components accounted for 91.9% of the variability. The external structures and the characteristics of the cell membranes in the different microbial groups are determinant for their susceptibility to essential oils and their major components