Rapid detection of anti-Vaccinia virus neutralizing antibodies
<p>Abstract</p> <p>Increasing infections with Monkeypox and Cowpox viruses pose a continuous and growing threat to human health. The standard method for detecting poxvirus neutralizing antibodies is the plaque-reduction neutralization test that is specific but also time-consuming a...
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| Format: | Article |
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BMC
2011-03-01
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| Series: | Virology Journal |
| Online Access: | http://www.virologyj.com/content/8/1/139 |
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Article |
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doaj-38e1c2c894b146e2842e21adb48b08342020-11-24T21:27:00ZengBMCVirology Journal1743-422X2011-03-018113910.1186/1743-422X-8-139Rapid detection of anti-Vaccinia virus neutralizing antibodiesLichtfuss Gregor FDrozd AnnaKramski MaritDabrowski Piotr WEllerbrok Heinz<p>Abstract</p> <p>Increasing infections with Monkeypox and Cowpox viruses pose a continuous and growing threat to human health. The standard method for detecting poxvirus neutralizing antibodies is the plaque-reduction neutralization test that is specific but also time-consuming and laborious. Therefore, a rapid and reliable method was developed to determine neutralizing antibody titers within twelve hours. The new assay measures viral mRNA transcription as a marker for actively replicating virus after incomplete neutralization using real-time PCR.</p> http://www.virologyj.com/content/8/1/139 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Lichtfuss Gregor F Drozd Anna Kramski Marit Dabrowski Piotr W Ellerbrok Heinz |
| spellingShingle |
Lichtfuss Gregor F Drozd Anna Kramski Marit Dabrowski Piotr W Ellerbrok Heinz Rapid detection of anti-Vaccinia virus neutralizing antibodies Virology Journal |
| author_facet |
Lichtfuss Gregor F Drozd Anna Kramski Marit Dabrowski Piotr W Ellerbrok Heinz |
| author_sort |
Lichtfuss Gregor F |
| title |
Rapid detection of anti-Vaccinia virus neutralizing antibodies |
| title_short |
Rapid detection of anti-Vaccinia virus neutralizing antibodies |
| title_full |
Rapid detection of anti-Vaccinia virus neutralizing antibodies |
| title_fullStr |
Rapid detection of anti-Vaccinia virus neutralizing antibodies |
| title_full_unstemmed |
Rapid detection of anti-Vaccinia virus neutralizing antibodies |
| title_sort |
rapid detection of anti-vaccinia virus neutralizing antibodies |
| publisher |
BMC |
| series |
Virology Journal |
| issn |
1743-422X |
| publishDate |
2011-03-01 |
| description |
<p>Abstract</p> <p>Increasing infections with Monkeypox and Cowpox viruses pose a continuous and growing threat to human health. The standard method for detecting poxvirus neutralizing antibodies is the plaque-reduction neutralization test that is specific but also time-consuming and laborious. Therefore, a rapid and reliable method was developed to determine neutralizing antibody titers within twelve hours. The new assay measures viral mRNA transcription as a marker for actively replicating virus after incomplete neutralization using real-time PCR.</p> |
| url |
http://www.virologyj.com/content/8/1/139 |
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