Stable expression and potential use of West Nile virus envelope glycoproteins preM/E as antigen in diagnostic tests
West Nile virus (WNV) envelope glycoproteins preM/E were stably expressed in baby hamster kidney cells and tested as antigen in a fluorescent antibody assay for WNV antibodies. Sera from horses, mice and chicken immunized with an inactivated WNV vaccine and, less consistently, sera from horses acute...
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Sociedade Brasileira de Microbiologia
2011-09-01
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| Series: | Brazilian Journal of Microbiology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300040 |
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doaj-3846737ecfb749fcad43dcbc820ea1232020-11-24T22:19:01ZengSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology1517-83821678-44052011-09-014231161116610.1590/S1517-83822011000300040Stable expression and potential use of West Nile virus envelope glycoproteins preM/E as antigen in diagnostic testsJuliana Felipetto CargneluttiMário Celso Sperotto BrumRudi WeiblenEduardo Furtado FloresWest Nile virus (WNV) envelope glycoproteins preM/E were stably expressed in baby hamster kidney cells and tested as antigen in a fluorescent antibody assay for WNV antibodies. Sera from horses, mice and chicken immunized with an inactivated WNV vaccine and, less consistently, sera from horses acutely infected with WNV, reacted specifically with viral antigens present in preM/E-expressing cells.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300040West Nile virusWNVM/E glycoproteinsstable expressiondiagnostic |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Juliana Felipetto Cargnelutti Mário Celso Sperotto Brum Rudi Weiblen Eduardo Furtado Flores |
| spellingShingle |
Juliana Felipetto Cargnelutti Mário Celso Sperotto Brum Rudi Weiblen Eduardo Furtado Flores Stable expression and potential use of West Nile virus envelope glycoproteins preM/E as antigen in diagnostic tests Brazilian Journal of Microbiology West Nile virus WNV M/E glycoproteins stable expression diagnostic |
| author_facet |
Juliana Felipetto Cargnelutti Mário Celso Sperotto Brum Rudi Weiblen Eduardo Furtado Flores |
| author_sort |
Juliana Felipetto Cargnelutti |
| title |
Stable expression and potential use of West Nile virus envelope glycoproteins preM/E as antigen in diagnostic tests |
| title_short |
Stable expression and potential use of West Nile virus envelope glycoproteins preM/E as antigen in diagnostic tests |
| title_full |
Stable expression and potential use of West Nile virus envelope glycoproteins preM/E as antigen in diagnostic tests |
| title_fullStr |
Stable expression and potential use of West Nile virus envelope glycoproteins preM/E as antigen in diagnostic tests |
| title_full_unstemmed |
Stable expression and potential use of West Nile virus envelope glycoproteins preM/E as antigen in diagnostic tests |
| title_sort |
stable expression and potential use of west nile virus envelope glycoproteins prem/e as antigen in diagnostic tests |
| publisher |
Sociedade Brasileira de Microbiologia |
| series |
Brazilian Journal of Microbiology |
| issn |
1517-8382 1678-4405 |
| publishDate |
2011-09-01 |
| description |
West Nile virus (WNV) envelope glycoproteins preM/E were stably expressed in baby hamster kidney cells and tested as antigen in a fluorescent antibody assay for WNV antibodies. Sera from horses, mice and chicken immunized with an inactivated WNV vaccine and, less consistently, sera from horses acutely infected with WNV, reacted specifically with viral antigens present in preM/E-expressing cells. |
| topic |
West Nile virus WNV M/E glycoproteins stable expression diagnostic |
| url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300040 |
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