Self-Assembled Thermoresponsive Nanogel from Grafted Hyaluronic Acid as a Biocompatible Delivery Platform for Curcumin with Enhanced Drug Loading and Biological Activities
A hyaluronic acid-grafted poly(<i>N</i>-isopropylacrylamide) (HA-pNIPAM) was synthesized as a polymeric nanogel platform for encapsulation and delivery of hydrophobic bioactive compounds using curcumin as a model drug. As demonstrated by transmission electron microscopy and dynamic light...
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doaj-383f7cf9823b49e6bcfda03dd1df1be12021-01-08T00:01:27ZengMDPI AGPolymers2073-43602021-01-011319419410.3390/polym13020194Self-Assembled Thermoresponsive Nanogel from Grafted Hyaluronic Acid as a Biocompatible Delivery Platform for Curcumin with Enhanced Drug Loading and Biological ActivitiesJittima Amie Luckanagul0Pahweenvaj Ratnatilaka Na Bhuket1Chawanphat Muangnoi2Pranee Rojsitthisak3Qian Wang4Pornchai Rojsitthisak5Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, ThailandNatural Products for Ageing and Chronic Diseases Research Unit, Chulalongkorn University, Bangkok 10330, ThailandCell and Animal Model Unit, Institute of Nutrition, Mahidol University, Nakhon Pathom 73170, ThailandNatural Products for Ageing and Chronic Diseases Research Unit, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Chemistry and Biochemistry, University of South Carolina, 631 Sumter St., Columbia, SC 29208, USANatural Products for Ageing and Chronic Diseases Research Unit, Chulalongkorn University, Bangkok 10330, ThailandA hyaluronic acid-grafted poly(<i>N</i>-isopropylacrylamide) (HA-pNIPAM) was synthesized as a polymeric nanogel platform for encapsulation and delivery of hydrophobic bioactive compounds using curcumin as a model drug. As demonstrated by transmission electron microscopy and dynamic light scattering techniques, the HA-pNIPAM was simply assembled into spherical nano-sized particles with the thermoresponsive behavior. The success of curcumin aqueous solubilization was confirmed by fluorescent spectroscopy. The resulting nanogel formulation enhanced the aqueous solubility and uptake into NIH-3T3 cells of curcumin. This nanogel formulation also demonstrates cytocompatibility against NIH-3T3 cells, which deems it safe as a delivery vehicle. Moreover, the formulation has a slight skin-protection effect using an artificial skin equivalence model. The curcumin-loaded HA-pNIPAM nanogel showed an anti-proliferative activity against MDA-MB-231, Caco-2, HepG2, HT-29, and TNF-α-induced hyperproliferation of keratinocyte (HaCaT) cells. The thermoresponsive HA-pNIPAM nanogel reported here could be further optimized as a platform for controlled-release systems to encapsulate pharmaceuticals for therapeutic applications.https://www.mdpi.com/2073-4360/13/2/194curcuminhyaluronic acidHA-pNIPAMthermoresponsive nanogel |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jittima Amie Luckanagul Pahweenvaj Ratnatilaka Na Bhuket Chawanphat Muangnoi Pranee Rojsitthisak Qian Wang Pornchai Rojsitthisak |
spellingShingle |
Jittima Amie Luckanagul Pahweenvaj Ratnatilaka Na Bhuket Chawanphat Muangnoi Pranee Rojsitthisak Qian Wang Pornchai Rojsitthisak Self-Assembled Thermoresponsive Nanogel from Grafted Hyaluronic Acid as a Biocompatible Delivery Platform for Curcumin with Enhanced Drug Loading and Biological Activities Polymers curcumin hyaluronic acid HA-pNIPAM thermoresponsive nanogel |
author_facet |
Jittima Amie Luckanagul Pahweenvaj Ratnatilaka Na Bhuket Chawanphat Muangnoi Pranee Rojsitthisak Qian Wang Pornchai Rojsitthisak |
author_sort |
Jittima Amie Luckanagul |
title |
Self-Assembled Thermoresponsive Nanogel from Grafted Hyaluronic Acid as a Biocompatible Delivery Platform for Curcumin with Enhanced Drug Loading and Biological Activities |
title_short |
Self-Assembled Thermoresponsive Nanogel from Grafted Hyaluronic Acid as a Biocompatible Delivery Platform for Curcumin with Enhanced Drug Loading and Biological Activities |
title_full |
Self-Assembled Thermoresponsive Nanogel from Grafted Hyaluronic Acid as a Biocompatible Delivery Platform for Curcumin with Enhanced Drug Loading and Biological Activities |
title_fullStr |
Self-Assembled Thermoresponsive Nanogel from Grafted Hyaluronic Acid as a Biocompatible Delivery Platform for Curcumin with Enhanced Drug Loading and Biological Activities |
title_full_unstemmed |
Self-Assembled Thermoresponsive Nanogel from Grafted Hyaluronic Acid as a Biocompatible Delivery Platform for Curcumin with Enhanced Drug Loading and Biological Activities |
title_sort |
self-assembled thermoresponsive nanogel from grafted hyaluronic acid as a biocompatible delivery platform for curcumin with enhanced drug loading and biological activities |
publisher |
MDPI AG |
series |
Polymers |
issn |
2073-4360 |
publishDate |
2021-01-01 |
description |
A hyaluronic acid-grafted poly(<i>N</i>-isopropylacrylamide) (HA-pNIPAM) was synthesized as a polymeric nanogel platform for encapsulation and delivery of hydrophobic bioactive compounds using curcumin as a model drug. As demonstrated by transmission electron microscopy and dynamic light scattering techniques, the HA-pNIPAM was simply assembled into spherical nano-sized particles with the thermoresponsive behavior. The success of curcumin aqueous solubilization was confirmed by fluorescent spectroscopy. The resulting nanogel formulation enhanced the aqueous solubility and uptake into NIH-3T3 cells of curcumin. This nanogel formulation also demonstrates cytocompatibility against NIH-3T3 cells, which deems it safe as a delivery vehicle. Moreover, the formulation has a slight skin-protection effect using an artificial skin equivalence model. The curcumin-loaded HA-pNIPAM nanogel showed an anti-proliferative activity against MDA-MB-231, Caco-2, HepG2, HT-29, and TNF-α-induced hyperproliferation of keratinocyte (HaCaT) cells. The thermoresponsive HA-pNIPAM nanogel reported here could be further optimized as a platform for controlled-release systems to encapsulate pharmaceuticals for therapeutic applications. |
topic |
curcumin hyaluronic acid HA-pNIPAM thermoresponsive nanogel |
url |
https://www.mdpi.com/2073-4360/13/2/194 |
work_keys_str_mv |
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