H3K27me3 Signal in the Cis Regulatory Elements Reveals the Differentiation Potential of Progenitors During Drosophila Neuroglial Development

Drosophila neural development undergoes extensive chromatin remodeling and precise epigenetic regulation. However, the roles of chromatin remodeling in establishment and maintenance of cell identity during cell fate transition remain enigmatic. Here, we compared the changes in gene expression, as we...

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Main Authors: Xiaolong Chen, Youqiong Ye, Liang Gu, Jin Sun, Yanhua Du, Wen-Ju Liu, Wei Li, Xiaobai Zhang, Cizhong Jiang
Format: Article
Language:English
Published: Elsevier 2019-06-01
Series:Genomics, Proteomics & Bioinformatics
Online Access:http://www.sciencedirect.com/science/article/pii/S1672022918301463
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spelling doaj-3818846e3c5e4ca79e0ee259f66ee1f52020-11-25T01:07:37ZengElsevierGenomics, Proteomics & Bioinformatics1672-02292019-06-01173297304H3K27me3 Signal in the Cis Regulatory Elements Reveals the Differentiation Potential of Progenitors During Drosophila Neuroglial DevelopmentXiaolong Chen0Youqiong Ye1Liang Gu2Jin Sun3Yanhua Du4Wen-Ju Liu5Wei Li6Xiaobai Zhang7Cizhong Jiang8Institute of Translational Research, Tongji Hospital, School of Life Sciences and Technology, Shanghai Key Laboratory of Signaling and Disease Research, Tongji University, Shanghai 200092, ChinaInstitute of Translational Research, Tongji Hospital, School of Life Sciences and Technology, Shanghai Key Laboratory of Signaling and Disease Research, Tongji University, Shanghai 200092, ChinaInstitute of Translational Research, Tongji Hospital, School of Life Sciences and Technology, Shanghai Key Laboratory of Signaling and Disease Research, Tongji University, Shanghai 200092, ChinaInstitute of Translational Research, Tongji Hospital, School of Life Sciences and Technology, Shanghai Key Laboratory of Signaling and Disease Research, Tongji University, Shanghai 200092, ChinaInstitute of Translational Research, Tongji Hospital, School of Life Sciences and Technology, Shanghai Key Laboratory of Signaling and Disease Research, Tongji University, Shanghai 200092, ChinaInstitute of Translational Research, Tongji Hospital, School of Life Sciences and Technology, Shanghai Key Laboratory of Signaling and Disease Research, Tongji University, Shanghai 200092, ChinaTongji University Library, Tongji University, Shanghai 200092, ChinaInstitute of Translational Research, Tongji Hospital, School of Life Sciences and Technology, Shanghai Key Laboratory of Signaling and Disease Research, Tongji University, Shanghai 200092, ChinaInstitute of Translational Research, Tongji Hospital, School of Life Sciences and Technology, Shanghai Key Laboratory of Signaling and Disease Research, Tongji University, Shanghai 200092, China; Research Center of Stem Cells and Ageing, Tsingtao Advanced Research Institute, Tongji University, Tsingtao 266071, China; Corresponding author.Drosophila neural development undergoes extensive chromatin remodeling and precise epigenetic regulation. However, the roles of chromatin remodeling in establishment and maintenance of cell identity during cell fate transition remain enigmatic. Here, we compared the changes in gene expression, as well as the dynamics of nucleosome positioning and key histone modifications between the four major neural cell types during Drosophila neural development. We find that the neural progenitors can be separated from the terminally differentiated cells based on their gene expression profiles, whereas nucleosome distribution in the flanking regions of transcription start sites fails to identify the relationships between the progenitors and the differentiated cells. H3K27me3 signal in promoters and enhancers can not only distinguish the progenitors from the differentiated cells but also identify the differentiation path of the neural stem cells (NSCs) to the intermediate progenitor cells to the glial cells. In contrast, H3K9ac signal fails to identify the differentiation path, although it activates distinct sets of genes with neuron-specific and glia-related functions during the differentiation of the NSCs into neurons and glia, respectively. Together, our study provides novel insights into the crucial roles of chromatin remodeling in determining cell type during Drosophila neural development. Keywords: Nucleosome, Histone modification, Neural stem cell, Neuron, Gliahttp://www.sciencedirect.com/science/article/pii/S1672022918301463
collection DOAJ
language English
format Article
sources DOAJ
author Xiaolong Chen
Youqiong Ye
Liang Gu
Jin Sun
Yanhua Du
Wen-Ju Liu
Wei Li
Xiaobai Zhang
Cizhong Jiang
spellingShingle Xiaolong Chen
Youqiong Ye
Liang Gu
Jin Sun
Yanhua Du
Wen-Ju Liu
Wei Li
Xiaobai Zhang
Cizhong Jiang
H3K27me3 Signal in the Cis Regulatory Elements Reveals the Differentiation Potential of Progenitors During Drosophila Neuroglial Development
Genomics, Proteomics & Bioinformatics
author_facet Xiaolong Chen
Youqiong Ye
Liang Gu
Jin Sun
Yanhua Du
Wen-Ju Liu
Wei Li
Xiaobai Zhang
Cizhong Jiang
author_sort Xiaolong Chen
title H3K27me3 Signal in the Cis Regulatory Elements Reveals the Differentiation Potential of Progenitors During Drosophila Neuroglial Development
title_short H3K27me3 Signal in the Cis Regulatory Elements Reveals the Differentiation Potential of Progenitors During Drosophila Neuroglial Development
title_full H3K27me3 Signal in the Cis Regulatory Elements Reveals the Differentiation Potential of Progenitors During Drosophila Neuroglial Development
title_fullStr H3K27me3 Signal in the Cis Regulatory Elements Reveals the Differentiation Potential of Progenitors During Drosophila Neuroglial Development
title_full_unstemmed H3K27me3 Signal in the Cis Regulatory Elements Reveals the Differentiation Potential of Progenitors During Drosophila Neuroglial Development
title_sort h3k27me3 signal in the cis regulatory elements reveals the differentiation potential of progenitors during drosophila neuroglial development
publisher Elsevier
series Genomics, Proteomics & Bioinformatics
issn 1672-0229
publishDate 2019-06-01
description Drosophila neural development undergoes extensive chromatin remodeling and precise epigenetic regulation. However, the roles of chromatin remodeling in establishment and maintenance of cell identity during cell fate transition remain enigmatic. Here, we compared the changes in gene expression, as well as the dynamics of nucleosome positioning and key histone modifications between the four major neural cell types during Drosophila neural development. We find that the neural progenitors can be separated from the terminally differentiated cells based on their gene expression profiles, whereas nucleosome distribution in the flanking regions of transcription start sites fails to identify the relationships between the progenitors and the differentiated cells. H3K27me3 signal in promoters and enhancers can not only distinguish the progenitors from the differentiated cells but also identify the differentiation path of the neural stem cells (NSCs) to the intermediate progenitor cells to the glial cells. In contrast, H3K9ac signal fails to identify the differentiation path, although it activates distinct sets of genes with neuron-specific and glia-related functions during the differentiation of the NSCs into neurons and glia, respectively. Together, our study provides novel insights into the crucial roles of chromatin remodeling in determining cell type during Drosophila neural development. Keywords: Nucleosome, Histone modification, Neural stem cell, Neuron, Glia
url http://www.sciencedirect.com/science/article/pii/S1672022918301463
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