In silico characterization of boron transporter (BOR1) protein sequences in Poaceae species

• Main Author: Ertuğrul Filiz
• Format: Article
• Language: English
• Published: Plovdiv University Press 2013-01-01
• Series: Journal of BioScience and Biotechnology
• Subjects: BOR1; Boron; in silico analysis; Poaceae; protein
• Online Access: http://www.jbb.uni-plovdiv.bg/documents/27807/59545/jbb_2013-2(2)-pages_137-144.pdf

Boron (B) is essential for the plant growth and development, and its primary function is connected with formation of the cell wall. Moreover, boron toxicity is a shared problem in semiarid and arid regions. In this study, boron transporter protein (BOR1) sequences from some Poaceae species (Hordeum vulgare subsp. vulgare, Zea mays, Brachypodium distachyon, Oryza sativa subsp. japonica, Oryza sativa subsp. indica, Sorghum bicolor, Triticum aestivum) were evaluated by bioinformatics tools. Physicochemical analyses revealed that most of BOR1 proteins were basic character and had generally aliphatic amino acids. Analysis of the domains showed that transmembrane domains were identified constantly and three motifs were detected with 50 amino acids length. Also, the motif SPNPWEPGSYDHWTVAKDMFNVPPAYIFGAFIPATMVAGLYYFDHSVASQ was found most frequently with 25 repeats. The phylogenetic tree showed divergence into two main clusters. B. distachyon species were clustered separately. Finally, this study contributes to the new BOR1 protein characterization in grasses and create scientific base for in silico analysis in future.