Effects of yeast extract and methyl jasmonate on the enhancement of solasodine biosynthesis in cell cultures of Solanum hainanense Hance

• Main Authors: NGUYEN HOANG LOC, NGUYEN HUU THUAN ANH, LE THI MINH KHUYEN, TON NU THUY AN
• Format: Article
• Language: English
• Published: Plovdiv University Press 2014-04-01
• Series: Journal of BioScience and Biotechnology
• Subjects: Cell suspension; methyl jasmonate; solasodine; Solanum hainanense
• Online Access: http://www.jbb.uni-plovdiv.bg/documents/27807/352484/jbb_2014-3(1)-pages_1-6.pdf/

In this work, the effects of the elicitors methyl jasmonate (MeJA) and yeast extract (YE) on the growth and solasodine production of Solanum hainanense cells were investigated. The results showed that various concentrations of MeJA (50-250 µM) and YE (1-4 g/L) have different eliciting influences. The increase of solasodine content induced by the elicitation of 3 g/L of YE and 50 µM of MeJA at the beginning of cell culture was about 1.9- and 1.3-fold, respectively, as compared with that of the non-elicitated cells. In general, YE (biotic elicitor) was more effective in enhancing solasodine production than MeJA (abiotic elicitor).